Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda

Lisa A. Mills; Joseph Kagaayi; Joseph P. Shott; Kevin Newell; John Baptist Bwanika; Victor Ssempijja; Simon Aluma; Thomas C. Quinn; Steven J. Reynolds; Ronald H. Gray

doi:10.1016/j.trstmh.2009.07.030

Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda

Lisa A. Mills, Joseph Kagaayi, Joseph P. Shott, Kevin Newell, John Baptist Bwanika, Victor Ssempijja, Simon Aluma, Thomas C. Quinn, Steven J. Reynolds, Ronald H. Gray

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

In this study, we report the performance of a prototype malaria rapid diagnostic test, Malaria F-test (MFT), compared with thick blood films from HIV-positive Ugandans undergoing malaria testing. In total, 21/154 samples (13.6%) were concordantly positive by both thick film and MFT and 129/154 samples (83.8%) were concordantly negative; 1 sample (0.6%) was thick film-positive but MFT-negative and 3 samples (1.9%) were thick film-negative but MFT-positive. The sensitivity of MFT was 95.5% (95% CI 77.2-99.9%) compared with thick film microscopy and the specificity was 97.7% (95% CI 93.5-99.5%). MFT was simple, rapid and effective for detection of Plasmodium falciparum among HIV-positive subjects in a rural, malaria-endemic African setting.

Original language	English (US)
Pages (from-to)	237-239
Number of pages	3
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	104
Issue number	3
DOIs	https://doi.org/10.1016/j.trstmh.2009.07.030
State	Published - Mar 2010

Keywords

Africa
Diagnosis
HIV/AIDS
Malaria
Rapid diagnostic test
Uganda

ASJC Scopus subject areas

Parasitology
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.trstmh.2009.07.030

Cite this

Mills, L. A., Kagaayi, J., Shott, J. P., Newell, K., Bwanika, J. B., Ssempijja, V., Aluma, S., Quinn, T. C., Reynolds, S. J., & Gray, R. H. (2010). Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda. Transactions of the Royal Society of Tropical Medicine and Hygiene, 104(3), 237-239. https://doi.org/10.1016/j.trstmh.2009.07.030

Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda. / Mills, Lisa A.; Kagaayi, Joseph; Shott, Joseph P. et al.

In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 104, No. 3, 03.2010, p. 237-239.

Research output: Contribution to journal › Article › peer-review

Mills, LA, Kagaayi, J, Shott, JP, Newell, K, Bwanika, JB, Ssempijja, V, Aluma, S, Quinn, TC , Reynolds, SJ & Gray, RH 2010, 'Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 104, no. 3, pp. 237-239. https://doi.org/10.1016/j.trstmh.2009.07.030

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abstract = "In this study, we report the performance of a prototype malaria rapid diagnostic test, Malaria F-test (MFT), compared with thick blood films from HIV-positive Ugandans undergoing malaria testing. In total, 21/154 samples (13.6%) were concordantly positive by both thick film and MFT and 129/154 samples (83.8%) were concordantly negative; 1 sample (0.6%) was thick film-positive but MFT-negative and 3 samples (1.9%) were thick film-negative but MFT-positive. The sensitivity of MFT was 95.5% (95% CI 77.2-99.9%) compared with thick film microscopy and the specificity was 97.7% (95% CI 93.5-99.5%). MFT was simple, rapid and effective for detection of Plasmodium falciparum among HIV-positive subjects in a rural, malaria-endemic African setting.",

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Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda

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Keywords

ASJC Scopus subject areas

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