Performance of a Commercial, Type-Specific Enzyme-Linked Immunosorbent Assay for Detection of Herpes Simplex Virus Type 2-Specific Antibodies in Ugandans

Oliver B. Laeyendecker, Charla Henson, Ronald H Gray, Ruby H N Nguyen, Bobbi Jo Horne, Maria J Wawer, David Serwadda, Noah Kiwanuka, Rhoda Ashley Morrow, Wayne Hogrefe, Thomas C Quinn

Research output: Contribution to journalArticle

Abstract

Two hundred forty-eight human immunodeficiency virus (HIV)-positive and 496 HIV-negative subjects in Uganda were tested by HerpeSelect herpes simplex virus type 2 enzyme-linked immunosorbent assay (ELISA) and Western blotting to optimize the ELISA for use in this population. A higher index cutoff value was required for optimal sensitivity and specificity, and overall performance of the assay was not affected by HIV status.

Original languageEnglish (US)
Pages (from-to)1794-1796
Number of pages3
JournalJournal of Clinical Microbiology
Volume42
Issue number4
DOIs
Publication statusPublished - Apr 2004

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ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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