Abstract
Perforin is a marker of functionally active cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. We examined perforin expression in endometrium throughout the menstrual cycle. In addition, perforin expression was studied in endometrial leukocytes in women with anovulatory cycles with or without progesterone therapy and in postmenopausal women. In the endometrium. perforin-positive endometrial lymphocytes increased in numbers in the middle through late secretory phases. In women with anovulatory cycles, the proliferative endometrium, before estrogen withdrawal endometrial break- down, contained less perforin-positive lymphocytes compared with the premenstrual endometrium of the normal menstrual cycle. The progestin-induced endometrial decidualization was accompanied by an extensive recruitment of perforin-positive cells. In contrast, the postmenopausal atrophic endometrium showed complete absence of perforin-positive cells. The lymphocytes isolated from secretory endometrium were CD3−, CD56+ (~80%) or CD3+, CD8+ T-cell receptor (TCR)-γδ− (~20%). Almost 90% ± 5.5 of the CD3− CD56+, and up to 25% ± 7% of CD8+ T lymphocytes contained perforin. Perforin expression w’as further confirmed in endometrial lymphocytes by Northern blot analysis. In vitro, isolated endometrial lymphocytes exhibited NK-like cytotoxicity. After cyclic hormone withdrawal during normal menstrual cycle, these perforin-positive cytotoxic cells may be involved in endometrial stromal breakdown during menstruation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 143-150 |
| Number of pages | 8 |
| Journal | International Journal of Gynecological Pathology |
| Volume | 14 |
| Issue number | 2 |
| State | Published - 1995 |
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Keywords
- Endometrial lymphocytes
- Immunohistochemistry
- Menstruation
- Northern blot
- Perforin
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Pathology and Forensic Medicine
Cite this
Perforin expression in endometrium during the menstrual cycle. / Hameed, Arif; Fox, William M.; Kurman, Robert J; Hruban, Ralph H; Podack, Eckhard R.
In: International Journal of Gynecological Pathology, Vol. 14, No. 2, 1995, p. 143-150.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Perforin expression in endometrium during the menstrual cycle
AU - Hameed, Arif
AU - Fox, William M.
AU - Kurman, Robert J
AU - Hruban, Ralph H
AU - Podack, Eckhard R.
PY - 1995
Y1 - 1995
N2 - Perforin is a marker of functionally active cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. We examined perforin expression in endometrium throughout the menstrual cycle. In addition, perforin expression was studied in endometrial leukocytes in women with anovulatory cycles with or without progesterone therapy and in postmenopausal women. In the endometrium. perforin-positive endometrial lymphocytes increased in numbers in the middle through late secretory phases. In women with anovulatory cycles, the proliferative endometrium, before estrogen withdrawal endometrial break- down, contained less perforin-positive lymphocytes compared with the premenstrual endometrium of the normal menstrual cycle. The progestin-induced endometrial decidualization was accompanied by an extensive recruitment of perforin-positive cells. In contrast, the postmenopausal atrophic endometrium showed complete absence of perforin-positive cells. The lymphocytes isolated from secretory endometrium were CD3−, CD56+ (~80%) or CD3+, CD8+ T-cell receptor (TCR)-γδ− (~20%). Almost 90% ± 5.5 of the CD3− CD56+, and up to 25% ± 7% of CD8+ T lymphocytes contained perforin. Perforin expression w’as further confirmed in endometrial lymphocytes by Northern blot analysis. In vitro, isolated endometrial lymphocytes exhibited NK-like cytotoxicity. After cyclic hormone withdrawal during normal menstrual cycle, these perforin-positive cytotoxic cells may be involved in endometrial stromal breakdown during menstruation.
AB - Perforin is a marker of functionally active cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. We examined perforin expression in endometrium throughout the menstrual cycle. In addition, perforin expression was studied in endometrial leukocytes in women with anovulatory cycles with or without progesterone therapy and in postmenopausal women. In the endometrium. perforin-positive endometrial lymphocytes increased in numbers in the middle through late secretory phases. In women with anovulatory cycles, the proliferative endometrium, before estrogen withdrawal endometrial break- down, contained less perforin-positive lymphocytes compared with the premenstrual endometrium of the normal menstrual cycle. The progestin-induced endometrial decidualization was accompanied by an extensive recruitment of perforin-positive cells. In contrast, the postmenopausal atrophic endometrium showed complete absence of perforin-positive cells. The lymphocytes isolated from secretory endometrium were CD3−, CD56+ (~80%) or CD3+, CD8+ T-cell receptor (TCR)-γδ− (~20%). Almost 90% ± 5.5 of the CD3− CD56+, and up to 25% ± 7% of CD8+ T lymphocytes contained perforin. Perforin expression w’as further confirmed in endometrial lymphocytes by Northern blot analysis. In vitro, isolated endometrial lymphocytes exhibited NK-like cytotoxicity. After cyclic hormone withdrawal during normal menstrual cycle, these perforin-positive cytotoxic cells may be involved in endometrial stromal breakdown during menstruation.
KW - Endometrial lymphocytes
KW - Immunohistochemistry
KW - Menstruation
KW - Northern blot
KW - Perforin
UR - http://www.scopus.com/inward/record.url?scp=0028945275&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028945275&partnerID=8YFLogxK
M3 - Article
C2 - 8601526
AN - SCOPUS:0028945275
VL - 14
SP - 143
EP - 150
JO - International Journal of Gynecological Pathology
JF - International Journal of Gynecological Pathology
SN - 0277-1691
IS - 2
ER -