Perforin expression in endometrium during the menstrual cycle

Arif Hameed, William M. Fox, Robert J Kurman, Ralph H Hruban, Eckhard R. Podack

Research output: Contribution to journalArticle

Abstract

Perforin is a marker of functionally active cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. We examined perforin expression in endometrium throughout the menstrual cycle. In addition, perforin expression was studied in endometrial leukocytes in women with anovulatory cycles with or without progesterone therapy and in postmenopausal women. In the endometrium. perforin-positive endometrial lymphocytes increased in numbers in the middle through late secretory phases. In women with anovulatory cycles, the proliferative endometrium, before estrogen withdrawal endometrial break- down, contained less perforin-positive lymphocytes compared with the premenstrual endometrium of the normal menstrual cycle. The progestin-induced endometrial decidualization was accompanied by an extensive recruitment of perforin-positive cells. In contrast, the postmenopausal atrophic endometrium showed complete absence of perforin-positive cells. The lymphocytes isolated from secretory endometrium were CD3, CD56+ (~80%) or CD3+, CD8+ T-cell receptor (TCR)-γδ (~20%). Almost 90% ± 5.5 of the CD3 CD56+, and up to 25% ± 7% of CD8+ T lymphocytes contained perforin. Perforin expression w’as further confirmed in endometrial lymphocytes by Northern blot analysis. In vitro, isolated endometrial lymphocytes exhibited NK-like cytotoxicity. After cyclic hormone withdrawal during normal menstrual cycle, these perforin-positive cytotoxic cells may be involved in endometrial stromal breakdown during menstruation.

Original languageEnglish (US)
Pages (from-to)143-150
Number of pages8
JournalInternational Journal of Gynecological Pathology
Volume14
Issue number2
StatePublished - 1995

Fingerprint

Perforin
Menstrual Cycle
Endometrium
Lymphocytes
Menstruation
Cytotoxic T-Lymphocytes
Progestins
T-Cell Antigen Receptor
Natural Killer Cells
Northern Blotting
Progesterone
Estrogens
Leukocytes
Hormones
T-Lymphocytes

Keywords

  • Endometrial lymphocytes
  • Immunohistochemistry
  • Menstruation
  • Northern blot
  • Perforin

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Pathology and Forensic Medicine

Cite this

Perforin expression in endometrium during the menstrual cycle. / Hameed, Arif; Fox, William M.; Kurman, Robert J; Hruban, Ralph H; Podack, Eckhard R.

In: International Journal of Gynecological Pathology, Vol. 14, No. 2, 1995, p. 143-150.

Research output: Contribution to journalArticle

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