"Perfect" designer chromosome v and behavior of a ring derivative

Ze Xiong Xie, Bing Zhi Li, Leslie A. Mitchell, Yi Wu, Xin Qi, Zhu Jin, Bin Jia, Xia Wang, Bo Xuan Zeng, Hui Min Liu, Xiao Le Wu, Qi Feng, Wen Zheng Zhang, Wei Liu, Ming Zhu Ding, Xia Li, Guang Rong Zhao, Jian Jun Qiao, Jing Sheng Cheng, Meng Zhao & 73 others Zheng Kuang, Xuya Wang, J. Andrew Martin, Giovanni Stracquadanio, Kun Yang, Xue Bai, Juan Zhao, Meng Long Hu, Qiu Hui Lin, Wen Qian Zhang, Ming Hua Shen, Si Chen, Wan Su, En Xu Wang, Rui Guo, Fang Zhai, Xue Jiao Guo, Hao Xing Du, Jia Qing Zhu, Tian Qing Song, Jun Jun Dai, Fei Fei Li, Guo Zhen Jiang, Shi Lei Han, Shi Yang Liu, Zhi Chao Yu, Xiao Na Yang, Ken Chen, Cheng Hu, Da Shuai Li, Nan Jia, Yue Liu, Lin Ting Wang, Su Wang, Xiao Tong Wei, Mei Qing Fu, Lan Meng Qu, Si Yu Xin, Ting Liu, Kai Ren Tian, Xue Nan Li, Jin Hua Zhang, Li Xiang Song, Jin Gui Liu, Jia Fei Lv, Hang Xu, Ran Tao, Yan Wang, Ting Ting Zhang, Ye Xuan Deng, Yi Ran Wang, Ting Li, Guang Xin Ye, Xiao Ran Xu, Zheng Bao Xia, Wei Zhang, Shi Lan Yang, Yi Lin Liu, Wen Qi Ding, Zhen Ning Liu, Jun Qi Zhu, Ning Zhi Liu, Roy Walker, Yisha Luo, Yun Wang, Yue Shen, Huanming Yang, Yizhi Cai, Ping Sheng Ma, Chun Ting Zhang, Joel S. Bader, Jef D. Boeke, Ying Jin Yuan

Research output: Contribution to journalArticle

Abstract

Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis.We designed and de novo synthesized 536, 024-base pair chromosome synV in the "Build-A-Genome China" course.We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in Saccharomyces cerevisiae under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.

Original languageEnglish (US)
Article numbereaaf4704
JournalScience
Volume355
Issue number6329
DOIs
StatePublished - Mar 10 2017

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Ring Chromosomes
Clustered Regularly Interspaced Short Palindromic Repeats
Chromosomes
Chromosome Disorders
Genome
Meiosis
Human Chromosomes
Spores
Base Pairing
Saccharomyces cerevisiae
China
Proteins

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Xie, Z. X., Li, B. Z., Mitchell, L. A., Wu, Y., Qi, X., Jin, Z., ... Yuan, Y. J. (2017). "Perfect" designer chromosome v and behavior of a ring derivative. Science, 355(6329), [eaaf4704]. https://doi.org/10.1126/science.aaf4704

"Perfect" designer chromosome v and behavior of a ring derivative. / Xie, Ze Xiong; Li, Bing Zhi; Mitchell, Leslie A.; Wu, Yi; Qi, Xin; Jin, Zhu; Jia, Bin; Wang, Xia; Zeng, Bo Xuan; Liu, Hui Min; Wu, Xiao Le; Feng, Qi; Zhang, Wen Zheng; Liu, Wei; Ding, Ming Zhu; Li, Xia; Zhao, Guang Rong; Qiao, Jian Jun; Cheng, Jing Sheng; Zhao, Meng; Kuang, Zheng; Wang, Xuya; Martin, J. Andrew; Stracquadanio, Giovanni; Yang, Kun; Bai, Xue; Zhao, Juan; Hu, Meng Long; Lin, Qiu Hui; Zhang, Wen Qian; Shen, Ming Hua; Chen, Si; Su, Wan; Wang, En Xu; Guo, Rui; Zhai, Fang; Guo, Xue Jiao; Du, Hao Xing; Zhu, Jia Qing; Song, Tian Qing; Dai, Jun Jun; Li, Fei Fei; Jiang, Guo Zhen; Han, Shi Lei; Liu, Shi Yang; Yu, Zhi Chao; Yang, Xiao Na; Chen, Ken; Hu, Cheng; Li, Da Shuai; Jia, Nan; Liu, Yue; Wang, Lin Ting; Wang, Su; Wei, Xiao Tong; Fu, Mei Qing; Qu, Lan Meng; Xin, Si Yu; Liu, Ting; Tian, Kai Ren; Li, Xue Nan; Zhang, Jin Hua; Song, Li Xiang; Liu, Jin Gui; Lv, Jia Fei; Xu, Hang; Tao, Ran; Wang, Yan; Zhang, Ting Ting; Deng, Ye Xuan; Wang, Yi Ran; Li, Ting; Ye, Guang Xin; Xu, Xiao Ran; Xia, Zheng Bao; Zhang, Wei; Yang, Shi Lan; Liu, Yi Lin; Ding, Wen Qi; Liu, Zhen Ning; Zhu, Jun Qi; Liu, Ning Zhi; Walker, Roy; Luo, Yisha; Wang, Yun; Shen, Yue; Yang, Huanming; Cai, Yizhi; Ma, Ping Sheng; Zhang, Chun Ting; Bader, Joel S.; Boeke, Jef D.; Yuan, Ying Jin.

In: Science, Vol. 355, No. 6329, eaaf4704, 10.03.2017.

Research output: Contribution to journalArticle

Xie, ZX, Li, BZ, Mitchell, LA, Wu, Y, Qi, X, Jin, Z, Jia, B, Wang, X, Zeng, BX, Liu, HM, Wu, XL, Feng, Q, Zhang, WZ, Liu, W, Ding, MZ, Li, X, Zhao, GR, Qiao, JJ, Cheng, JS, Zhao, M, Kuang, Z, Wang, X, Martin, JA, Stracquadanio, G, Yang, K, Bai, X, Zhao, J, Hu, ML, Lin, QH, Zhang, WQ, Shen, MH, Chen, S, Su, W, Wang, EX, Guo, R, Zhai, F, Guo, XJ, Du, HX, Zhu, JQ, Song, TQ, Dai, JJ, Li, FF, Jiang, GZ, Han, SL, Liu, SY, Yu, ZC, Yang, XN, Chen, K, Hu, C, Li, DS, Jia, N, Liu, Y, Wang, LT, Wang, S, Wei, XT, Fu, MQ, Qu, LM, Xin, SY, Liu, T, Tian, KR, Li, XN, Zhang, JH, Song, LX, Liu, JG, Lv, JF, Xu, H, Tao, R, Wang, Y, Zhang, TT, Deng, YX, Wang, YR, Li, T, Ye, GX, Xu, XR, Xia, ZB, Zhang, W, Yang, SL, Liu, YL, Ding, WQ, Liu, ZN, Zhu, JQ, Liu, NZ, Walker, R, Luo, Y, Wang, Y, Shen, Y, Yang, H, Cai, Y, Ma, PS, Zhang, CT, Bader, JS, Boeke, JD & Yuan, YJ 2017, '"Perfect" designer chromosome v and behavior of a ring derivative', Science, vol. 355, no. 6329, eaaf4704. https://doi.org/10.1126/science.aaf4704
Xie ZX, Li BZ, Mitchell LA, Wu Y, Qi X, Jin Z et al. "Perfect" designer chromosome v and behavior of a ring derivative. Science. 2017 Mar 10;355(6329). eaaf4704. https://doi.org/10.1126/science.aaf4704
Xie, Ze Xiong ; Li, Bing Zhi ; Mitchell, Leslie A. ; Wu, Yi ; Qi, Xin ; Jin, Zhu ; Jia, Bin ; Wang, Xia ; Zeng, Bo Xuan ; Liu, Hui Min ; Wu, Xiao Le ; Feng, Qi ; Zhang, Wen Zheng ; Liu, Wei ; Ding, Ming Zhu ; Li, Xia ; Zhao, Guang Rong ; Qiao, Jian Jun ; Cheng, Jing Sheng ; Zhao, Meng ; Kuang, Zheng ; Wang, Xuya ; Martin, J. Andrew ; Stracquadanio, Giovanni ; Yang, Kun ; Bai, Xue ; Zhao, Juan ; Hu, Meng Long ; Lin, Qiu Hui ; Zhang, Wen Qian ; Shen, Ming Hua ; Chen, Si ; Su, Wan ; Wang, En Xu ; Guo, Rui ; Zhai, Fang ; Guo, Xue Jiao ; Du, Hao Xing ; Zhu, Jia Qing ; Song, Tian Qing ; Dai, Jun Jun ; Li, Fei Fei ; Jiang, Guo Zhen ; Han, Shi Lei ; Liu, Shi Yang ; Yu, Zhi Chao ; Yang, Xiao Na ; Chen, Ken ; Hu, Cheng ; Li, Da Shuai ; Jia, Nan ; Liu, Yue ; Wang, Lin Ting ; Wang, Su ; Wei, Xiao Tong ; Fu, Mei Qing ; Qu, Lan Meng ; Xin, Si Yu ; Liu, Ting ; Tian, Kai Ren ; Li, Xue Nan ; Zhang, Jin Hua ; Song, Li Xiang ; Liu, Jin Gui ; Lv, Jia Fei ; Xu, Hang ; Tao, Ran ; Wang, Yan ; Zhang, Ting Ting ; Deng, Ye Xuan ; Wang, Yi Ran ; Li, Ting ; Ye, Guang Xin ; Xu, Xiao Ran ; Xia, Zheng Bao ; Zhang, Wei ; Yang, Shi Lan ; Liu, Yi Lin ; Ding, Wen Qi ; Liu, Zhen Ning ; Zhu, Jun Qi ; Liu, Ning Zhi ; Walker, Roy ; Luo, Yisha ; Wang, Yun ; Shen, Yue ; Yang, Huanming ; Cai, Yizhi ; Ma, Ping Sheng ; Zhang, Chun Ting ; Bader, Joel S. ; Boeke, Jef D. ; Yuan, Ying Jin. / "Perfect" designer chromosome v and behavior of a ring derivative. In: Science. 2017 ; Vol. 355, No. 6329.
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abstract = "Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis.We designed and de novo synthesized 536, 024-base pair chromosome synV in the {"}Build-A-Genome China{"} course.We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in Saccharomyces cerevisiae under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.",
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AU - Xie, Ze Xiong

AU - Li, Bing Zhi

AU - Mitchell, Leslie A.

AU - Wu, Yi

AU - Qi, Xin

AU - Jin, Zhu

AU - Jia, Bin

AU - Wang, Xia

AU - Zeng, Bo Xuan

AU - Liu, Hui Min

AU - Wu, Xiao Le

AU - Feng, Qi

AU - Zhang, Wen Zheng

AU - Liu, Wei

AU - Ding, Ming Zhu

AU - Li, Xia

AU - Zhao, Guang Rong

AU - Qiao, Jian Jun

AU - Cheng, Jing Sheng

AU - Zhao, Meng

AU - Kuang, Zheng

AU - Wang, Xuya

AU - Martin, J. Andrew

AU - Stracquadanio, Giovanni

AU - Yang, Kun

AU - Bai, Xue

AU - Zhao, Juan

AU - Hu, Meng Long

AU - Lin, Qiu Hui

AU - Zhang, Wen Qian

AU - Shen, Ming Hua

AU - Chen, Si

AU - Su, Wan

AU - Wang, En Xu

AU - Guo, Rui

AU - Zhai, Fang

AU - Guo, Xue Jiao

AU - Du, Hao Xing

AU - Zhu, Jia Qing

AU - Song, Tian Qing

AU - Dai, Jun Jun

AU - Li, Fei Fei

AU - Jiang, Guo Zhen

AU - Han, Shi Lei

AU - Liu, Shi Yang

AU - Yu, Zhi Chao

AU - Yang, Xiao Na

AU - Chen, Ken

AU - Hu, Cheng

AU - Li, Da Shuai

AU - Jia, Nan

AU - Liu, Yue

AU - Wang, Lin Ting

AU - Wang, Su

AU - Wei, Xiao Tong

AU - Fu, Mei Qing

AU - Qu, Lan Meng

AU - Xin, Si Yu

AU - Liu, Ting

AU - Tian, Kai Ren

AU - Li, Xue Nan

AU - Zhang, Jin Hua

AU - Song, Li Xiang

AU - Liu, Jin Gui

AU - Lv, Jia Fei

AU - Xu, Hang

AU - Tao, Ran

AU - Wang, Yan

AU - Zhang, Ting Ting

AU - Deng, Ye Xuan

AU - Wang, Yi Ran

AU - Li, Ting

AU - Ye, Guang Xin

AU - Xu, Xiao Ran

AU - Xia, Zheng Bao

AU - Zhang, Wei

AU - Yang, Shi Lan

AU - Liu, Yi Lin

AU - Ding, Wen Qi

AU - Liu, Zhen Ning

AU - Zhu, Jun Qi

AU - Liu, Ning Zhi

AU - Walker, Roy

AU - Luo, Yisha

AU - Wang, Yun

AU - Shen, Yue

AU - Yang, Huanming

AU - Cai, Yizhi

AU - Ma, Ping Sheng

AU - Zhang, Chun Ting

AU - Bader, Joel S.

AU - Boeke, Jef D.

AU - Yuan, Ying Jin

PY - 2017/3/10

Y1 - 2017/3/10

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