Percentage binding of testosterone, androstenedione and dehydroisoandrosterone in human plasma

Maguelone G. Forest, Marco A. Rivarola, Claude J. Migeon

Research output: Contribution to journalArticlepeer-review

Abstract

Equilibrium dialysis, ultrafiltration by centrifugation and by vacuum gave similar results for the percentage binding of testosterone (T), androstenedione (Δ) and dehydroisoandrosterone (D) when using plasma samples diluted 1:5 at 37°C. The percentage binding of T is significantly lower in male plasma than in female plasma. Pregnancy and estrogen treatment in males increase T binding, whereas ProveraRtreatment in males decreases it. In addition to T itself, 5α-androstan-3α, 17β-diol, estradiol-17β and to a smaller extent estrone can compete for T binding. The percentage binding of Δ was similar in male and female plasma. Pregnancy and estrogen or ProveraR treatment in males had no effect. In range of concentration used, there was no binding competition by the steroid tested. Although the percentage binding of D was not influenced by sex, it was increased in pregnant women and estrogen-treated men. There was also a slight competition by T.

Original languageEnglish (US)
Pages (from-to)323-343
Number of pages21
JournalSteroids
Volume12
Issue number3
DOIs
StatePublished - Sep 1968

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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