Peptidomic Analysis of Maternal Serum to Identify Biomarker Candidates for Prenatal Diagnosis of Tetralogy of Fallot

Bin Zhuang, Yin Hu, Xuemei Fan, Mengmeng Li, Jingai Zhu, Heng Liu, Li Cao, Dong Liang, Jingjing Zhang, Zhangbin Yu, Shuping Han

Research output: Contribution to journalArticlepeer-review

Abstract

Tetralogy of Fallot (TOF) is the most common form of cyanotic congenital heart disease. To identify endogenous peptides possibly involved in the progression of TOF, we performed comparative peptidomic profiling of maternal serum between normal fetuses and fetuses suffering from TOF. A total of 278 differentially expressed peptides, including 94 over-expressed peptides and 184 under-expressed peptides, originating from 227 protein precursors were identified by liquid chromatography/mass spectrometry (LC/MS) in maternal serum of fetuses with TOF compared to normal controls. Further, ingenuity pathway analysis (IPA) was used to identify putative roles for these peptides in cardiovascular development. Two peptides were derived from functional domains of proteins involved in heart development and associated with TOF; these may represent candidate bioactive peptides involved in TOF. These peptides may be related to the pathologic changes in the heart associated with TOF, and may be useful as novel biomarkers for prenatal diagnosis of TOF. J. Cell. Biochem. 119: 468–477, 2018.

Original languageEnglish (US)
Pages (from-to)468-477
Number of pages10
JournalJournal of cellular biochemistry
Volume119
Issue number1
DOIs
StatePublished - Jan 2018
Externally publishedYes

Keywords

  • BIOMARKER
  • MATERNAL SERUM
  • PEPTIDOMIC ANALYSIS
  • TETRALOGY OF FALLOT

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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