@article{8f75a5e80daa46de80e91f550a921bb1,
title = "Peptidomic Analysis of Maternal Serum to Identify Biomarker Candidates for Prenatal Diagnosis of Tetralogy of Fallot",
abstract = "Tetralogy of Fallot (TOF) is the most common form of cyanotic congenital heart disease. To identify endogenous peptides possibly involved in the progression of TOF, we performed comparative peptidomic profiling of maternal serum between normal fetuses and fetuses suffering from TOF. A total of 278 differentially expressed peptides, including 94 over-expressed peptides and 184 under-expressed peptides, originating from 227 protein precursors were identified by liquid chromatography/mass spectrometry (LC/MS) in maternal serum of fetuses with TOF compared to normal controls. Further, ingenuity pathway analysis (IPA) was used to identify putative roles for these peptides in cardiovascular development. Two peptides were derived from functional domains of proteins involved in heart development and associated with TOF; these may represent candidate bioactive peptides involved in TOF. These peptides may be related to the pathologic changes in the heart associated with TOF, and may be useful as novel biomarkers for prenatal diagnosis of TOF. J. Cell. Biochem. 119: 468–477, 2018.",
keywords = "BIOMARKER, MATERNAL SERUM, PEPTIDOMIC ANALYSIS, TETRALOGY OF FALLOT",
author = "Bin Zhuang and Yin Hu and Xuemei Fan and Mengmeng Li and Jingai Zhu and Heng Liu and Li Cao and Dong Liang and Jingjing Zhang and Zhangbin Yu and Shuping Han",
note = "Funding Information: Abbreviations used: ACN, acetonitrile; CHD, congenital heart disease; IPA, ingenuity pathway analysis; kDa, kilo Dalton; LC/MS, liquid chromatography/mass spectrometry; LMW, low molecular weight; RV, (right ventricular) outflow tract obstruction; RVH, right ventricular hypertrophy; TOF, Tetralogy of Fallot; VSD, ventricular septal defect. Bin Zhuang and Yin Hu contributed equally to this work. Disclosure statement: The authors have declared no conflict of interest. Grant sponsor: Nanjing Medical Science and Technique Development Foundation; Grant number: 201605052; Grant sponsor: National Natural Science Foundation of Jiangsu Province of China; Grant number: BK20141077; Grant sponsor: National Natural Science Foundation of China; Grant number: 81370200. *Correspondence to: Prof. Shuping Han and Prof. Zhangbin Yu, Department of Pediatrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, 123rd Tian Fei Xiang, Mo Chou Road, Nanjing 210004, Jiangsu Province, China. E-mails: shupinghan@njmu.edu.cn (S.H.) and zhangbinyu@njmu.edu.cn (Z.Y.) Manuscript Received: 20 April 2017; Manuscript Accepted: 8 June 2017 Accepted manuscript online in Wiley Online Library (wileyonlinelibrary.com): 9 June 2017 DOI 10.1002/jcb.26204 • {\textcopyright} 2017 Wiley Periodicals, Inc. Funding Information: This study was supported by grants from the National Natural Science Foundation of China (grant no. 81370200), the National Natural Science Foundation of Jiangsu Province of China (No. BK20141077), and the Nanjing Medical Science and Technique Development Foundation (No. 201605052). Publisher Copyright: {\textcopyright} 2017 Wiley Periodicals, Inc.",
year = "2018",
month = jan,
doi = "10.1002/jcb.26204",
language = "English (US)",
volume = "119",
pages = "468--477",
journal = "Journal of cellular biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "1",
}