Peptides presented to the immune system by the murine class II major histocompatibility complex molecule I-A

Donald F. Hunt, Hanspeter Michel, Tracey A. Dickinson, Jeffrey Shabanowitz, Andrea Cox, Kazuyasu Sakaguchi, Ettore Appella, Howard M. Grey, Alessandro Sette

Research output: Contribution to journalArticle

Abstract

Between 650 and 2000 different peptides are associated with the major histocompatibility complex class Il molecule I-Ad. Sequences for nine of these were obtained by a combination of automated Edman degradation and tandem mass spectrometry. All of the peptides are derived from secretory or integral membrane proteins that are synthesized by the antigen-presenting cell itself. Peptides were 16 to 18 residues long, had ragged NH2- and COOH-termini, and contained a six-residue binding motif that was variably placed within the peptide chain. Binding data on truncated peptides suggest that the peptide binding groove on class II molecules can be open at both ends.

Original languageEnglish (US)
Pages (from-to)1817-1820
Number of pages4
JournalScience
Volume256
Issue number5065
StatePublished - Jun 26 1992
Externally publishedYes

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Major Histocompatibility Complex
Immune System
Peptides
Antigen-Presenting Cells
Tandem Mass Spectrometry
Membrane Proteins

ASJC Scopus subject areas

  • General

Cite this

Hunt, D. F., Michel, H., Dickinson, T. A., Shabanowitz, J., Cox, A., Sakaguchi, K., ... Sette, A. (1992). Peptides presented to the immune system by the murine class II major histocompatibility complex molecule I-A. Science, 256(5065), 1817-1820.

Peptides presented to the immune system by the murine class II major histocompatibility complex molecule I-A. / Hunt, Donald F.; Michel, Hanspeter; Dickinson, Tracey A.; Shabanowitz, Jeffrey; Cox, Andrea; Sakaguchi, Kazuyasu; Appella, Ettore; Grey, Howard M.; Sette, Alessandro.

In: Science, Vol. 256, No. 5065, 26.06.1992, p. 1817-1820.

Research output: Contribution to journalArticle

Hunt, DF, Michel, H, Dickinson, TA, Shabanowitz, J, Cox, A, Sakaguchi, K, Appella, E, Grey, HM & Sette, A 1992, 'Peptides presented to the immune system by the murine class II major histocompatibility complex molecule I-A', Science, vol. 256, no. 5065, pp. 1817-1820.
Hunt DF, Michel H, Dickinson TA, Shabanowitz J, Cox A, Sakaguchi K et al. Peptides presented to the immune system by the murine class II major histocompatibility complex molecule I-A. Science. 1992 Jun 26;256(5065):1817-1820.
Hunt, Donald F. ; Michel, Hanspeter ; Dickinson, Tracey A. ; Shabanowitz, Jeffrey ; Cox, Andrea ; Sakaguchi, Kazuyasu ; Appella, Ettore ; Grey, Howard M. ; Sette, Alessandro. / Peptides presented to the immune system by the murine class II major histocompatibility complex molecule I-A. In: Science. 1992 ; Vol. 256, No. 5065. pp. 1817-1820.
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