Peptide sequencing using MALDI on time-of-flight and ion trap mass spectrometers

Robert J. Cotter, Timothy J. Cornish, Marcela Cordero, Vladimir M. Doroshenko

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Two instruments have been developed in our laboratory for the direct amino-acid sequencing of peptides. The first is a time-of-flight (TOF) mass spectrometer that utilizes a coaxial, curved-field reflectron which provides simultaneous focusing of product ins formed by post- source decay (PSD) and obviates the need for scanning or stepping the reflectron voltage. The second is a matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometer (ITMS). In this instrument, MALDI ions are trapped efficiently by ramping the trapping rf field, and a linear mass scale is achieved by modulating the axial excitation voltage during mass scanning. Monoisotopic mass selection and excitation is accomplished using phase- modulated SWIFT (stored waveform inverse Fourier transform) techniques, while efficient CID (collision-induced dissociation) performance is achieved using pulsed introduction of Xe and other heavier gases. Both mass spectrometers have been designed as compact instruments for sequencing peptides in partially fractionated mixtures.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsGerald E. Cohn, Steven A. Soper, C.H.W. Chen
Pages366-377
Number of pages12
StatePublished - Jan 1 1996
EventUltrasensitive Biochemical Diagnostics - San Jose, CA, USA
Duration: Jan 31 1996Feb 2 1996

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2680
ISSN (Print)0277-786X

Other

OtherUltrasensitive Biochemical Diagnostics
CitySan Jose, CA, USA
Period1/31/962/2/96

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Peptide sequencing using MALDI on time-of-flight and ion trap mass spectrometers'. Together they form a unique fingerprint.

Cite this