Peptide release on the ribosome depends critically on the 2' OH of the peptidyl tRNA substrate

Julie L. Brunelle, Jeffrey J. Shaw, Elaine M. Youngman, Rachel Green

Research output: Contribution to journalArticlepeer-review

Abstract

Peptide release on the ribosome is catalyzed by protein release factors (RFs) on recognition of stop codons positioned in the A site of the small ribosomal subunit. Here we show that the 2′ OH of the peptidyl-tRNA substrate plays an essential role in catalysis of the peptide release reaction. These observations parallel earlier studies of the mechanism of the peptidyl transfer reaction and argue that related mechanisms are at the heart of catalysis for these reactions.

Original languageEnglish (US)
Pages (from-to)1526-1531
Number of pages6
JournalRNA
Volume14
Issue number8
DOIs
StatePublished - Aug 2008

Keywords

  • Release factor
  • Ribosome
  • Translation termination

ASJC Scopus subject areas

  • Molecular Biology

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