Abstract
The Na+-H+ exchanger regulatory factor (NHE-RF) is a cytoplasmic phosphoprotein that was first found to be involved in protein kinase A mediated regulation of ion transport. NHE-RF contains two distinct protein interaction PDZ domains: NHE-RF-PDZ1 and NHE-RB-PDZ2. However, their binding partners are currently unknown, Because PDZ domains usually bind to specific short linear C-terminal sequences, we have carried out affinity selection of random peptides for specific sequences that interact with the NHE-RF PDZ domains and found that NHE-RF-PDZ1 is capable of binding to the CFTR C-terminus. The specific and tight association suggests a potential regulatory role of NHE-RF in cystic fibrosis transmembrane conductance regulator (CFTR) function.
Original language | English (US) |
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Pages (from-to) | 103-108 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 427 |
Issue number | 1 |
DOIs | |
State | Published - May 1 1998 |
Keywords
- Cystic fibrosis
- PDZ domain
- PKA 1
- Peptide display
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology