Peptide-β2-microglobulin-MHC fusion molecules bind antigen-specific T cells and can be used for multivalent MHC-Ig complexes

Tim F. Greten, Firouzeh Korangy, Gunnar Neumann, Heiner Wedemeyer, Karola Schlote, Astrid Heller, Stephan Scheffer, Drew M. Pardoll, Annette I. Garbe, Jonathan P. Schneck, Michael P. Manns

Research output: Contribution to journalArticlepeer-review

Abstract

Recombinant soluble MHC molecules are widely used for visualization, activation and inhibition of antigen-specific immune responses. Using a genetic approach, we have generated two novel peptide-β2-microglobulin-MHC constructs. We have linked the MHC molecule with the peptide of interest, without limiting the recognition by the cognate TCR. This molecule can also be joined with the IgG heavy chain resulting in a dimeric MHC-Ig fusion protein. These molecules bind antigen-specific T cells with high specificity and sensitivity, therefore, providing a valuable tool for detection as well as enrichment of antigen-specific T cells.

Original languageEnglish (US)
Pages (from-to)125-135
Number of pages11
JournalJournal of Immunological Methods
Volume271
Issue number1-2
DOIs
StatePublished - Dec 20 2002

Keywords

  • CD8+ T cell
  • Multimeric MHC

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Peptide-β2-microglobulin-MHC fusion molecules bind antigen-specific T cells and can be used for multivalent MHC-Ig complexes'. Together they form a unique fingerprint.

Cite this