@article{db32363a4bc34805bcb6197a35936aa2,
title = "Peptide-β2-microglobulin-MHC fusion molecules bind antigen-specific T cells and can be used for multivalent MHC-Ig complexes",
abstract = "Recombinant soluble MHC molecules are widely used for visualization, activation and inhibition of antigen-specific immune responses. Using a genetic approach, we have generated two novel peptide-β2-microglobulin-MHC constructs. We have linked the MHC molecule with the peptide of interest, without limiting the recognition by the cognate TCR. This molecule can also be joined with the IgG heavy chain resulting in a dimeric MHC-Ig fusion protein. These molecules bind antigen-specific T cells with high specificity and sensitivity, therefore, providing a valuable tool for detection as well as enrichment of antigen-specific T cells.",
keywords = "CD8+ T cell, Multimeric MHC",
author = "Greten, {Tim F.} and Firouzeh Korangy and Gunnar Neumann and Heiner Wedemeyer and Karola Schlote and Astrid Heller and Stephan Scheffer and Pardoll, {Drew M.} and Garbe, {Annette I.} and Schneck, {Jonathan P.} and Manns, {Michael P.}",
note = "Funding Information: The HHH sequence was kindly provided by B{\'e}atrice P{\'e}rarnau, Paris, a human β2-microglobulin cDNA sequence by Mark Soloski and the Tax-specific T cell clone by Bill Biddison, NIH. Recombinant human IL-2 was a gift from Dr. Wagner, Chiron Therapeutics. Support for this work was provided by grants from the NIH AI-29575 and AI-44129 to Jonathan Schneck. T.G. was supported by the Deutsche Forschungsgemeinschaft (Gr1511/2-1), Hochschulinterne Leistungsf{\"o}rderung of the Medizinische Hochschule Hannover and Wilhem Sander Stiftung. Under licensing agreements between Pharmingen and Johns Hopkins University, Dr. Schneck and Dr. Greten are entitled to a share of the royalty received by the University on sales of DimerX products. Dr. Schneck is also a paid consultant to Pharmingen. The terms of this agreement are being managed by the Johns Hopkins University in accordance with its conflict of interest policies. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",
year = "2002",
month = dec,
day = "20",
doi = "10.1016/S0022-1759(02)00346-0",
language = "English (US)",
volume = "271",
pages = "125--135",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1-2",
}