PEGylation of nanoparticles improves their cytoplasmic transport

Junghae Suh, Kok Leong Choy, Samuel K. Lai, Jung Soo Suk, Benjamin C. Tang, Sudhir Prabhu, Justin Hanes

Research output: Contribution to journalArticle

Abstract

The efficacy of nucleus-targeted drug- or gene-carrying nanoparticles may be limited by slow transport through the molecularly crowded cytoplasm following endosome escape. Cytoskeletal elements and cellular organelles may pose steric and/or adhesive obstacles to the efficient intracellular transport of nanoparticles. To potentially reduce adhesive interactions of colloids with intracellular components, the surface of model nanoparticles was coated with polyethylene glycol (PEG). Subsequently, multiple-particle tracking (MPT) was used to quantify the cytoplasmic transport rates of particles microinjected into the cytoplasm of live cells. PEGylation increased average nanoparticle diffusivities by 100% compared to unPEGylated particles (time scale of 10 s) in live cells. Faster particle transport correlated with a marked decrease in the number of particles that underwent hindered transport, from 79.2% (unmodified) to 48.8% (PEGylated). This result adds to an impressive list of positive benefits associated with PEGylation of drug and gene delivery vectors.

Original languageEnglish (US)
Pages (from-to)735-741
Number of pages7
JournalInternational journal of nanomedicine
Volume2
Issue number4
StatePublished - Dec 1 2007

Keywords

  • Cytoplasm
  • Microinjection
  • PEG
  • Particle tracking
  • Polystyrene

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

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  • Cite this

    Suh, J., Choy, K. L., Lai, S. K., Suk, J. S., Tang, B. C., Prabhu, S., & Hanes, J. (2007). PEGylation of nanoparticles improves their cytoplasmic transport. International journal of nanomedicine, 2(4), 735-741.