Abstract
Inbred mouse strains have demonstrated a range of susceptibilities to inhaled environmental irritants. C57Bl/6J mice are highly susceptible while C3H/HeJ mice are resistant to ozone exposures, as assessed by lavaged protein. However, lavaged protein reflects a loss of both the endothelial and epithelial barrier. To determine whether basal differences exist in the epithelial barrier, we measured soluble tracer (99mtechnetium-diethylenetriamine pentaacetic acid, 99mTc-DTPA) clearance from the lung in spontaneously breathing, anesthetized mice and mice ventilated with increased lung volume with applied positive end-expiratory pressure (PEEP; 1, 6, or 10 cmH2O). Both strains showed more rapid clearance during ventilation with 10 cmH2O PEEP compared with other ventilation pressures (p <0.001). There was a substantial difference in clearance between the two strains during ventilation with 10 cmH2O PEEP (mean half time for C57Bl/6J mice = 19 ± 4 min versus 34 ± 3 min for C3H/HeJ mice; p <0.001). Thus, when lung volume is increased, the susceptible C57Bl/6J strain shows a greater change in epithelial barrier than the resistant C3H/HeJ strain. These results may reflect fundamental differences in lung architecture.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 340-344 |
| Number of pages | 5 |
| Journal | Respiratory Physiology and Neurobiology |
| Volume | 156 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 15 2007 |
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Keywords
- Clearance
- Positive end-expiratory pressure
ASJC Scopus subject areas
- Physiology
- Pulmonary and Respiratory Medicine
- Neuroscience(all)
Cite this
PEEP-induced changes in epithelial permeability in inbred mouse strains. / Bishai, John M.; Mitzner, Wayne A; Tankersley, Clarke G.; Wagner, Elizabeth Marie.
In: Respiratory Physiology and Neurobiology, Vol. 156, No. 3, 15.06.2007, p. 340-344.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - PEEP-induced changes in epithelial permeability in inbred mouse strains
AU - Bishai, John M.
AU - Mitzner, Wayne A
AU - Tankersley, Clarke G.
AU - Wagner, Elizabeth Marie
PY - 2007/6/15
Y1 - 2007/6/15
N2 - Inbred mouse strains have demonstrated a range of susceptibilities to inhaled environmental irritants. C57Bl/6J mice are highly susceptible while C3H/HeJ mice are resistant to ozone exposures, as assessed by lavaged protein. However, lavaged protein reflects a loss of both the endothelial and epithelial barrier. To determine whether basal differences exist in the epithelial barrier, we measured soluble tracer (99mtechnetium-diethylenetriamine pentaacetic acid, 99mTc-DTPA) clearance from the lung in spontaneously breathing, anesthetized mice and mice ventilated with increased lung volume with applied positive end-expiratory pressure (PEEP; 1, 6, or 10 cmH2O). Both strains showed more rapid clearance during ventilation with 10 cmH2O PEEP compared with other ventilation pressures (p <0.001). There was a substantial difference in clearance between the two strains during ventilation with 10 cmH2O PEEP (mean half time for C57Bl/6J mice = 19 ± 4 min versus 34 ± 3 min for C3H/HeJ mice; p <0.001). Thus, when lung volume is increased, the susceptible C57Bl/6J strain shows a greater change in epithelial barrier than the resistant C3H/HeJ strain. These results may reflect fundamental differences in lung architecture.
AB - Inbred mouse strains have demonstrated a range of susceptibilities to inhaled environmental irritants. C57Bl/6J mice are highly susceptible while C3H/HeJ mice are resistant to ozone exposures, as assessed by lavaged protein. However, lavaged protein reflects a loss of both the endothelial and epithelial barrier. To determine whether basal differences exist in the epithelial barrier, we measured soluble tracer (99mtechnetium-diethylenetriamine pentaacetic acid, 99mTc-DTPA) clearance from the lung in spontaneously breathing, anesthetized mice and mice ventilated with increased lung volume with applied positive end-expiratory pressure (PEEP; 1, 6, or 10 cmH2O). Both strains showed more rapid clearance during ventilation with 10 cmH2O PEEP compared with other ventilation pressures (p <0.001). There was a substantial difference in clearance between the two strains during ventilation with 10 cmH2O PEEP (mean half time for C57Bl/6J mice = 19 ± 4 min versus 34 ± 3 min for C3H/HeJ mice; p <0.001). Thus, when lung volume is increased, the susceptible C57Bl/6J strain shows a greater change in epithelial barrier than the resistant C3H/HeJ strain. These results may reflect fundamental differences in lung architecture.
KW - Clearance
KW - Positive end-expiratory pressure
UR - http://www.scopus.com/inward/record.url?scp=33947237937&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33947237937&partnerID=8YFLogxK
U2 - 10.1016/j.resp.2006.10.009
DO - 10.1016/j.resp.2006.10.009
M3 - Article
C2 - 17134945
AN - SCOPUS:33947237937
VL - 156
SP - 340
EP - 344
JO - Respiratory Physiology and Neurobiology
JF - Respiratory Physiology and Neurobiology
SN - 1569-9048
IS - 3
ER -