@article{d5670a31ecd240da8a70db900fbba5df,
title = "PDZD7-MYO7A complex identified in enriched stereocilia membranes",
abstract = "While more than 70 genes have been linked to deafness, most of which are expressed in mechanosensory hair cells of the inner ear, a challenge has been to link these genes into molecular pathways. One example is Myo7a (myosin VIIA), in which deafness mutations affect the development and function of the mechanically sensitive stereocilia of hair cells. We describe here a procedure for the isolation of low-abundance protein complexes from stereocilia membrane fractions. Using this procedure, combined with identification and quantitation of proteins with mass spectrometry, we demonstrate that MYO7A forms a complex with PDZD7, a paralog of USH1C and DFNB31. MYO7A and PDZD7 interact in tissue-culture cells, and co-localize to the ankle-link region of stereocilia in wild-type but not Myo7a mutant mice. Our data thus describe a new paradigm for the interrogation of low-abundance protein complexes in hair cell stereocilia and establish an unanticipated link between MYO7A and PDZD7.",
author = "Morgan, {Clive P.} and Krey, {Jocelyn F.} and M{\textquoteright}hamed Grati and Bo Zhao and Shannon Fallen and Abhiraami Kannan-Sundhari and Liu, {Xue Zhong} and Dongseok Choi and Ulrich M{\"u}ller and Barr-Gillespie, {Peter G.}",
note = "Funding Information: We received support from the following core facilities: mass spectrometry from the OHSU Proteomics Shared Resource (partial support from NIH core grants P30 EY010572 & P30 CA069533; Orbitrap Fusion S10 OD012246), hybridoma cell culture and antibody production from the VGTI Monoclonal Antibody Core (OHSU), and confocal microscopy from the OHSU Advanced Light Microscopy Core @ The Jungers Center. The 138-1 hybridoma was developed by DJ Orten and T Hasson and was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242. National Institute on Deafness and Other Communication Disorders F32DC012455 Jocelyn F Krey National Institute on Deafness and Other Communication Disorders R03DC014544 Jocelyn F Krey National Institute on Deafness and Other Communication Disorders R01DC05575 Xue Zhong Liu National Institute on Deafness and Other Communication Disorders R01DC012546 Xue Zhong Liu National Institute on Deafness and Other Communication Disorders R01DC012115 Xue Zhong Liu National Institute on Deafness and Other Communication Disorders R01DC014427 Ulrich M{\"u}ller Peter G Barr-Gillespie National Institute on Deafnessand Other Communication Disorders R01DC005965 Ulrich M{\"u}ller National Institute on Deafness and Other Communication Disorders R01DC002368 Peter G Barr-Gillespie National Institute on Deafness and Other Communication Disorders P30DC005983 Peter G Barr-Gillespie Publisher Copyright: {\textcopyright} Morgan et al.",
year = "2016",
month = aug,
day = "15",
doi = "10.7554/eLife.18312",
language = "English (US)",
volume = "5",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
number = "AUGUST",
}