PDGF-C induces maturation of blood vessels in a model of glioblastoma and attenuates the response to anti-VEGF treatment

Emmanuelle di Tomaso, Nyall London, Daniel Fuja, James Logie, James A. Tyrrell, Walid Kamoun, Lance L. Munn, Rakesh K. Jain

Research output: Contribution to journalArticle

Abstract

Background: Recent clinical trials of VEGF inhibitors have shown promise in the treatment of recurrent glioblastomas (GBM). However, the survival benefit is usually short-lived as tumors escape anti-VEGF therapies. Here we tested the hypothesis that Platelet Derived Growth Factor-C (PDGF-C), an isoform of the PDGF family, affects GBM progression independent of VEGF pathway and hinders anti-VEGF therapy. Principal Findings: We first showed that PDGF-C is present in human GBMs. Then, we overexpressed or downregulated PDGF-C in a human GBM cell line, U87MG, and grew them in cranial windows in nude mice to assess vessel structure and function using intravital microscopy. PDGF-C overexpressing tumors had smaller vessel diameters and lower vascular permeability compared to the parental or siRNA-transfected tumors. Furthermore, vessels in PDGF-C overexpressing tumors had more extensive coverage with NG2 positive perivascular cells and a thicker collagen IV basement membrane than the controls. Treatment with DC101, an anti-VEGFR-2 antibody, induced decreases in vessel density in the parental tumors, but had no effect on the PDGF-C overexpressing tumors. Conclusion: These results suggest that PDGF-C plays an important role in glioma vessel maturation and stabilization, and that it can attenuate the response to anti-VEGF therapy, potentially contributing to escape from vascular normalization.

Original languageEnglish (US)
Article numbere5123
JournalPloS one
Volume4
Issue number4
DOIs
StatePublished - Apr 8 2009
Externally publishedYes

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platelet-derived growth factor
Blood vessels
Glioblastoma
blood vessels
Vascular Endothelial Growth Factor A
Blood Vessels
Tumors
neoplasms
Neoplasms
therapeutics
vascular endothelial growth factor receptor-2
Tumor Escape
Vascular Endothelial Growth Factor Receptor-2
human cell lines
basement membrane
Capillary Permeability
small interfering RNA
platelet-derived growth factor C
Basement Membrane
Nude Mice

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

PDGF-C induces maturation of blood vessels in a model of glioblastoma and attenuates the response to anti-VEGF treatment. / di Tomaso, Emmanuelle; London, Nyall; Fuja, Daniel; Logie, James; Tyrrell, James A.; Kamoun, Walid; Munn, Lance L.; Jain, Rakesh K.

In: PloS one, Vol. 4, No. 4, e5123, 08.04.2009.

Research output: Contribution to journalArticle

di Tomaso, Emmanuelle ; London, Nyall ; Fuja, Daniel ; Logie, James ; Tyrrell, James A. ; Kamoun, Walid ; Munn, Lance L. ; Jain, Rakesh K. / PDGF-C induces maturation of blood vessels in a model of glioblastoma and attenuates the response to anti-VEGF treatment. In: PloS one. 2009 ; Vol. 4, No. 4.
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