PD-L1 expression comparison between primary and relapsed non-small cell lung carcinoma using whole sections and clone SP263

Enrico Munari, Giuseppe Zamboni, Gianluigi Lunardi, Marcella Marconi, Marco Sommaggio, Matteo Brunelli, Guido Martignoni, George J. Netto, Mohammad O. Hoque, Francesca Moretta, Maria Cristina Mingari, Maria Cristina Pegoraro, Francesca Romana Mariotti, Paola Vacca, Lorenzo Moretta, Giuseppe Bogina

Research output: Contribution to journalArticlepeer-review

Abstract

We assessed the concordance, in terms of PD-L1 expression, between primary and metastatic non-small cell lung carcinoma (NSCLC) of different histotypes using validated SP263 clone. A few samples of local recurrences have also been analyzed. Whole sections of consecutive cases of primary NSCLC and paired relapses undergone surgical resection have been stained with PD-L1 clone SP263; for scoring purposes, a three-tiered system was applied using the following thresholds: <1%, 1-49% and ≥50%. Eighty-four cases of paired primary and relapsed tumors from 83 patients were analyzed, including 75 metastases and 9 local recurrences. Regarding metastases, when considering a cutoff of 1%, discrepancy in PD-L1 expression occurred in 9/75 (12%) paired samples (kappa value = 0.75); at 50% cutoff, discrepancy in PD-L1 expression was detected in 7/75 (9.3%) of paired samples (kappa value = 0.61). Regarding recurrences, at 1% cutoff, the discrepancy in PD-L1 expression was seen in 3/9 (33%) paired samples and in all cases there was a gained PD-L1 expression; at 50% cutoff, 1/9 (11%) paired samples showed gained PD-L1 expression. Our data provide important information regarding the concordance between primary and relapsed NSCLC and the degree of reliability of metastatic sites in terms of PD-L1 expression evaluation.

Original languageEnglish (US)
Pages (from-to)30465-30471
Number of pages7
JournalOncotarget
Volume9
Issue number54
DOIs
StatePublished - Jul 1 2018

Keywords

  • Cancer
  • Heterogeneity
  • Lung
  • Metastases
  • PD-L1

ASJC Scopus subject areas

  • Oncology

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