PD-1 ligands expressed on myeloid-derived APC in the CNS regulate T-cell responses in EAE

Bettina Schreiner, Samantha L. Bailey, Tahiro Shin, Lieping Chen, Stephen D. Miller

Research output: Contribution to journalArticlepeer-review

Abstract

Disease progression in experimental autoimmune encephalomyelitis (EAE) is regulated by programmed death receptor 1 (PD-1) and its ligands, B7-H1 (programmed death ligand 1 (PD-L1)) and B7-DC (PD-L2). B7-H1 and B7-DC have negative regulatory effects upon binding PD-1 on activated T cells and B7-H1 deficiency increases severity of both diabetes and EAE. However, the role of PD-L expression on different APC in the CNS in regulating local T-cell function during relapsing EAE has not been examined. Our data show that the majority of CNS CD4+ T cells isolated during acute EAE are PD-1+, and T cells specific for relapse-associated epitopes express PD-1 upon antigen stimulation in the CNS. B7-H1 and B7-DC are differentially expressed on discrete APC populations in the inflamed CNS. B7-H1 and PD-1 have mainly inhibitory functions on CNS T cells. B7-H1 negatively regulates the stimulation of activated PD-1+ TH cells, in co-cultures with microglia and different CNS-infiltrating APC presenting endogenously processed peptides. The preponderance of IFN-γ+ versus IL-17+ T cells in the CNS of B7-H1-/- mice suggests that B7-H1 more selectively suppresses TH-1 than TH-17 responses in vivo. In contrast, blockade of B7-DC has less pronounced regulatory effects. Overall, the results demonstrate that B7-H1 expressed by CNS myeloid APC negatively regulates T-cell activation during acute relapsing EAE.

Original languageEnglish (US)
Pages (from-to)2706-2717
Number of pages12
JournalEuropean Journal of Immunology
Volume38
Issue number10
DOIs
StatePublished - 2008

Keywords

  • Antigen presenting cells
  • Autoimmunity
  • Costimulatory molecules
  • Dendritic cells
  • Inhibitory receptors

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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