TY - JOUR
T1 - “PCR karyotype” of monochromosomal somatic cell hybrids
AU - Ning, Yi
AU - Lovell, Mercedes
AU - Cooley, Linda D.
AU - Pereira-Smith, Olivia M.
PY - 1993/6
Y1 - 1993/6
N2 - Following fusion of human diploid fibroblast-derived microcells with mouse A9 cells, we isolated seven monochromosomal hybrids containing a single human chromosome 2, 3, 5, 12, 15, 20, or 22. Cytogenetic analysis as well as PCR karyotyping (chromosome-specific banding pattern generated by Alu-PCR) was performed on all the hybrids. We here present, for the first time, the specific PCR karyotypes of human chromosomes 2 and 20.
AB - Following fusion of human diploid fibroblast-derived microcells with mouse A9 cells, we isolated seven monochromosomal hybrids containing a single human chromosome 2, 3, 5, 12, 15, 20, or 22. Cytogenetic analysis as well as PCR karyotyping (chromosome-specific banding pattern generated by Alu-PCR) was performed on all the hybrids. We here present, for the first time, the specific PCR karyotypes of human chromosomes 2 and 20.
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U2 - 10.1006/geno.1993.1260
DO - 10.1006/geno.1993.1260
M3 - Article
C2 - 8325652
AN - SCOPUS:0027210417
SN - 0888-7543
VL - 16
SP - 758
EP - 760
JO - Genomics
JF - Genomics
IS - 3
ER -