Pb2+ exposure alters the lens αA-crystallin protein profile in vivo and induces cataract formation in lens organ culture

R. Neal, N. Aykin-Burns, N. Ercal, J. S. Zigler

Research output: Contribution to journalArticle

Abstract

Epidemiological data supports lead exposure as a risk factor for cataract development. Previous studies which demonstrated oxidative imbalances in the lens following in vivo Pb2+ exposure support the idea that lead exposure can alter the lens biochemical homeostasis which may ultimately lead to loss of lens clarity with time. α-Crystallin, a major lens structural protein and molecular chaperone, undergoes various post-translational modifications upon aging which may contribute to decreased chaperone function and contribute to loss of lens clarity. This study evaluated the impact of 5 weeks of oral Pb2+ exposure (peripheral Pb2+ level ∼30 μg/dL) on the αA-crystallin protein profile of the lens from Fisher 344 rats. Decreases in relative protein spot intensity of more acidic forms of αA- and βA4-crystallin and of truncated forms of αA-crystallin were noted. This data indicates that changes in post-translational processing of crystallins do occur in vivo following short courses of clinically relevant Pb2+-exposure. In addition, organ culture of lenses from 4.5-month-old rats in 5 μM Pb2+ resulted in opacities, demonstrating that lead is toxic to the lens and can induce a loss of lens clarity.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalToxicology
Volume212
Issue number1
DOIs
StatePublished - Aug 15 2005
Externally publishedYes

Fingerprint

Crystallins
Organ Culture Techniques
Cataract
Lenses
Proteins
Rats
Molecular Chaperones
Poisons
Post Translational Protein Processing
Opacity
Homeostasis
Aging of materials

Keywords

  • α-Crystallin
  • Cataract
  • Lens
  • Pb

ASJC Scopus subject areas

  • Toxicology

Cite this

Pb2+ exposure alters the lens αA-crystallin protein profile in vivo and induces cataract formation in lens organ culture. / Neal, R.; Aykin-Burns, N.; Ercal, N.; Zigler, J. S.

In: Toxicology, Vol. 212, No. 1, 15.08.2005, p. 1-9.

Research output: Contribution to journalArticle

Neal, R. ; Aykin-Burns, N. ; Ercal, N. ; Zigler, J. S. / Pb2+ exposure alters the lens αA-crystallin protein profile in vivo and induces cataract formation in lens organ culture. In: Toxicology. 2005 ; Vol. 212, No. 1. pp. 1-9.
@article{b60db1c255d748b1ba11b9001313e175,
title = "Pb2+ exposure alters the lens αA-crystallin protein profile in vivo and induces cataract formation in lens organ culture",
abstract = "Epidemiological data supports lead exposure as a risk factor for cataract development. Previous studies which demonstrated oxidative imbalances in the lens following in vivo Pb2+ exposure support the idea that lead exposure can alter the lens biochemical homeostasis which may ultimately lead to loss of lens clarity with time. α-Crystallin, a major lens structural protein and molecular chaperone, undergoes various post-translational modifications upon aging which may contribute to decreased chaperone function and contribute to loss of lens clarity. This study evaluated the impact of 5 weeks of oral Pb2+ exposure (peripheral Pb2+ level ∼30 μg/dL) on the αA-crystallin protein profile of the lens from Fisher 344 rats. Decreases in relative protein spot intensity of more acidic forms of αA- and βA4-crystallin and of truncated forms of αA-crystallin were noted. This data indicates that changes in post-translational processing of crystallins do occur in vivo following short courses of clinically relevant Pb2+-exposure. In addition, organ culture of lenses from 4.5-month-old rats in 5 μM Pb2+ resulted in opacities, demonstrating that lead is toxic to the lens and can induce a loss of lens clarity.",
keywords = "α-Crystallin, Cataract, Lens, Pb",
author = "R. Neal and N. Aykin-Burns and N. Ercal and Zigler, {J. S.}",
year = "2005",
month = "8",
day = "15",
doi = "10.1016/j.tox.2005.03.015",
language = "English (US)",
volume = "212",
pages = "1--9",
journal = "Toxicology",
issn = "0300-483X",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Pb2+ exposure alters the lens αA-crystallin protein profile in vivo and induces cataract formation in lens organ culture

AU - Neal, R.

AU - Aykin-Burns, N.

AU - Ercal, N.

AU - Zigler, J. S.

PY - 2005/8/15

Y1 - 2005/8/15

N2 - Epidemiological data supports lead exposure as a risk factor for cataract development. Previous studies which demonstrated oxidative imbalances in the lens following in vivo Pb2+ exposure support the idea that lead exposure can alter the lens biochemical homeostasis which may ultimately lead to loss of lens clarity with time. α-Crystallin, a major lens structural protein and molecular chaperone, undergoes various post-translational modifications upon aging which may contribute to decreased chaperone function and contribute to loss of lens clarity. This study evaluated the impact of 5 weeks of oral Pb2+ exposure (peripheral Pb2+ level ∼30 μg/dL) on the αA-crystallin protein profile of the lens from Fisher 344 rats. Decreases in relative protein spot intensity of more acidic forms of αA- and βA4-crystallin and of truncated forms of αA-crystallin were noted. This data indicates that changes in post-translational processing of crystallins do occur in vivo following short courses of clinically relevant Pb2+-exposure. In addition, organ culture of lenses from 4.5-month-old rats in 5 μM Pb2+ resulted in opacities, demonstrating that lead is toxic to the lens and can induce a loss of lens clarity.

AB - Epidemiological data supports lead exposure as a risk factor for cataract development. Previous studies which demonstrated oxidative imbalances in the lens following in vivo Pb2+ exposure support the idea that lead exposure can alter the lens biochemical homeostasis which may ultimately lead to loss of lens clarity with time. α-Crystallin, a major lens structural protein and molecular chaperone, undergoes various post-translational modifications upon aging which may contribute to decreased chaperone function and contribute to loss of lens clarity. This study evaluated the impact of 5 weeks of oral Pb2+ exposure (peripheral Pb2+ level ∼30 μg/dL) on the αA-crystallin protein profile of the lens from Fisher 344 rats. Decreases in relative protein spot intensity of more acidic forms of αA- and βA4-crystallin and of truncated forms of αA-crystallin were noted. This data indicates that changes in post-translational processing of crystallins do occur in vivo following short courses of clinically relevant Pb2+-exposure. In addition, organ culture of lenses from 4.5-month-old rats in 5 μM Pb2+ resulted in opacities, demonstrating that lead is toxic to the lens and can induce a loss of lens clarity.

KW - α-Crystallin

KW - Cataract

KW - Lens

KW - Pb

UR - http://www.scopus.com/inward/record.url?scp=20444366189&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444366189&partnerID=8YFLogxK

U2 - 10.1016/j.tox.2005.03.015

DO - 10.1016/j.tox.2005.03.015

M3 - Article

C2 - 15905016

AN - SCOPUS:20444366189

VL - 212

SP - 1

EP - 9

JO - Toxicology

JF - Toxicology

SN - 0300-483X

IS - 1

ER -