Abstract
The kinetics of blood and organ engraftment following transplants of defined populations of hematopoietic stem/progenitor cells were investigated utilizing cell populations defined by surface antigen and rhodamine-123 staining. While long-term repopulating stem cells, short-term multipotent progenitors and committed progenitors all reconstituted peripheral blood red cells and splenic cellularity, only the population of cells that includes highly enriched long-term repopulating stem cells (Thy-1.1(low)Lin(neg)Sca- 1+Rh123(low)) reconstituted marrow cellularity. In addition, peripheral blood platelet and nucleated cell count increased only after transplant of the long-term repopulating population. These results argue that the major cell population that functions to reconstitute hematopoiesis after bone marrow transplantation is a primitive, marrow-homing stem cell. Transplantation of highly enriched multipotent progenitors that lack long- term reconstituting potential had no impact on hematopoietic recovery, apart from a transient increase in circulating erythrocytes. These results suggest that the primary cell population that functions to reconstitute hematopoiesis in a transplant setting is the long-term repopulating stem cell. This observation is discussed in the context of the normal hematopoietic process.
Original language | English (US) |
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Pages (from-to) | 31-39 |
Number of pages | 9 |
Journal | Stem Cells |
Volume | 15 |
Issue number | SUPPL. 1 |
State | Published - 1997 |
Externally published | Yes |
Keywords
- Bone marrow transplantation
- Engraftment kinetics
- Hematopoiesis
- Progenitor cells
- Stem cell biology
ASJC Scopus subject areas
- Cell Biology