Patterns of organ-specific engraftment by stem cell subsets and committed progenitors

W. E. Nibley, S. J. Pohlmann, G. J. Spangrude, E. D. Zanjani, H. E. Broxmeyer, S. J. Sharkis, B. Torok-Storb, J. W. Adamson, W. E. Fibbe

Research output: Contribution to journalArticlepeer-review

Abstract

The kinetics of blood and organ engraftment following transplants of defined populations of hematopoietic stem/progenitor cells were investigated utilizing cell populations defined by surface antigen and rhodamine-123 staining. While long-term repopulating stem cells, short-term multipotent progenitors and committed progenitors all reconstituted peripheral blood red cells and splenic cellularity, only the population of cells that includes highly enriched long-term repopulating stem cells (Thy-1.1(low)Lin(neg)Sca- 1+Rh123(low)) reconstituted marrow cellularity. In addition, peripheral blood platelet and nucleated cell count increased only after transplant of the long-term repopulating population. These results argue that the major cell population that functions to reconstitute hematopoiesis after bone marrow transplantation is a primitive, marrow-homing stem cell. Transplantation of highly enriched multipotent progenitors that lack long- term reconstituting potential had no impact on hematopoietic recovery, apart from a transient increase in circulating erythrocytes. These results suggest that the primary cell population that functions to reconstitute hematopoiesis in a transplant setting is the long-term repopulating stem cell. This observation is discussed in the context of the normal hematopoietic process.

Original languageEnglish (US)
Pages (from-to)31-39
Number of pages9
JournalStem Cells
Volume15
Issue numberSUPPL. 1
StatePublished - 1997
Externally publishedYes

Keywords

  • Bone marrow transplantation
  • Engraftment kinetics
  • Hematopoiesis
  • Progenitor cells
  • Stem cell biology

ASJC Scopus subject areas

  • Cell Biology

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