Patterns and extent of isotype-specificity in the murine H chain switch DNA rearrangement

Cynthia Schultz, John Petrini, John Collins, J. Latham Claflin, Kathleen A. Denis, Patricia Gearhart, Christine Gritzmacher, Tim Manser, Marc Shulman, Wesley Dunnick

Research output: Contribution to journalArticle

Abstract

We have analyzed the configuration of the H chain locus of 41 hybridomas by Southern blot analysis. Each H chain switch region was determined to be germ line, rearranged, or deleted. Including 13 previously analyzed hybridomas, 60% of those with rearrangements on both alleles showed a correlation of the two alleles, i.e., both the expressed and the nonexpressed alleles have rearranged to the same H chain constant region gene segment When the two H chain alleles did not rearrange to the same gene, they often rearranged to neighboring H chain genes. These results support a role for isotype-specific factors in H chain switch recombination The action of these isotype-specific factors may be propagated to some extent along the chromosome, which would lead to rearrangements to neighboring genes.

Original languageEnglish (US)
Pages (from-to)363-370
Number of pages8
JournalJournal of Immunology
Volume144
Issue number1
StatePublished - Jan 1 1990

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Gene Rearrangement
Alleles
Hybridomas
Genes
Southern Blotting
Germ Cells
Genetic Recombination
Chromosomes

ASJC Scopus subject areas

  • Immunology

Cite this

Schultz, C., Petrini, J., Collins, J., Claflin, J. L., Denis, K. A., Gearhart, P., ... Dunnick, W. (1990). Patterns and extent of isotype-specificity in the murine H chain switch DNA rearrangement. Journal of Immunology, 144(1), 363-370.

Patterns and extent of isotype-specificity in the murine H chain switch DNA rearrangement. / Schultz, Cynthia; Petrini, John; Collins, John; Claflin, J. Latham; Denis, Kathleen A.; Gearhart, Patricia; Gritzmacher, Christine; Manser, Tim; Shulman, Marc; Dunnick, Wesley.

In: Journal of Immunology, Vol. 144, No. 1, 01.01.1990, p. 363-370.

Research output: Contribution to journalArticle

Schultz, C, Petrini, J, Collins, J, Claflin, JL, Denis, KA, Gearhart, P, Gritzmacher, C, Manser, T, Shulman, M & Dunnick, W 1990, 'Patterns and extent of isotype-specificity in the murine H chain switch DNA rearrangement', Journal of Immunology, vol. 144, no. 1, pp. 363-370.
Schultz C, Petrini J, Collins J, Claflin JL, Denis KA, Gearhart P et al. Patterns and extent of isotype-specificity in the murine H chain switch DNA rearrangement. Journal of Immunology. 1990 Jan 1;144(1):363-370.
Schultz, Cynthia ; Petrini, John ; Collins, John ; Claflin, J. Latham ; Denis, Kathleen A. ; Gearhart, Patricia ; Gritzmacher, Christine ; Manser, Tim ; Shulman, Marc ; Dunnick, Wesley. / Patterns and extent of isotype-specificity in the murine H chain switch DNA rearrangement. In: Journal of Immunology. 1990 ; Vol. 144, No. 1. pp. 363-370.
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