Patterns and extent of isotype-specificity in the murine H chain switch DNA rearrangement

C. Schultz, J. Petrini, J. Collins, J. L. Claflin, K. A. Denis, P. Gearhart, C. Gritzmacher, T. Manser, M. Shulman, W. Dunnick

Research output: Contribution to journalArticlepeer-review

Abstract

We have analyzed the configuration of the H chain locus of 41 hybridomas by Southern blot analysis. Each H chain switch region was determined to be germ line, rearranged, or deleted. Including 13 previously analyzed hybridomas, 60% of those with rearrangements on both alleles showed a correlation of the two alleles, i.e. both the expressed and the nonexpressed alleles have rearranged to the same H chain constant region gene segment. When the two H chain alleles did not rearrange to the same gene, they often rearranged to neighboring H chain genes. These results support a role for isotype-specific factors in H chain switch recombination. The action of these isotype-specific factors may be propagated to some extent along the chromosome, which would lead to rearrangements to neighboring genes.

Original languageEnglish (US)
Pages (from-to)363-370
Number of pages8
JournalJournal of Immunology
Volume144
Issue number1
StatePublished - 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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