TY - JOUR
T1 - Pathogenicity of Mycobacterium avium for human monocytes
T2 - Absence of macrophage-activating factor activity of gamma interferon
AU - Toba, H.
AU - Crawford, J. T.
AU - Ellner, J. J.
PY - 1989
Y1 - 1989
N2 - Mycobacterium avium is a frequent opportunistic pathogen in the acquired immunodeficiency syndrome (AIDS). We compared 12 strains of M. avium in an in vitro model of pathogenicity. Peripheral blood-derived monocytes from healthy individuals were infected with M. avium in vitro. Bacterial uptake and intracellular replication were assessed by microscopic count of acid-fast bacilli and CFU of bacteria, respectively, in lysed monocytes. The CFU assay showed that among five AIDS-associated strains, only one replicated in monocytes. Two of seven non-AIDS-associated strains replicated intracellularly. In addition, we examined the effect of gamma interferon (IFN-γ) on M. avium infection. IFN-γ treatment of monocytes decreased phagocytosis and had no effect on the intracellular replication of M. avium. Thus, most strains of M. avium do not multiply within monocytes from healthy individuals and IFN-γ does not have macrophage-activating factor activity for M. avium infection of human monocytes.
AB - Mycobacterium avium is a frequent opportunistic pathogen in the acquired immunodeficiency syndrome (AIDS). We compared 12 strains of M. avium in an in vitro model of pathogenicity. Peripheral blood-derived monocytes from healthy individuals were infected with M. avium in vitro. Bacterial uptake and intracellular replication were assessed by microscopic count of acid-fast bacilli and CFU of bacteria, respectively, in lysed monocytes. The CFU assay showed that among five AIDS-associated strains, only one replicated in monocytes. Two of seven non-AIDS-associated strains replicated intracellularly. In addition, we examined the effect of gamma interferon (IFN-γ) on M. avium infection. IFN-γ treatment of monocytes decreased phagocytosis and had no effect on the intracellular replication of M. avium. Thus, most strains of M. avium do not multiply within monocytes from healthy individuals and IFN-γ does not have macrophage-activating factor activity for M. avium infection of human monocytes.
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M3 - Article
C2 - 2491838
AN - SCOPUS:0024537159
SN - 0019-9567
VL - 57
SP - 239
EP - 244
JO - Infection and Immunity
JF - Infection and Immunity
IS - 1
ER -