TY - JOUR
T1 - Pathogenesis of ovine lentivirus-induced arthritis
T2 - Phenotypic evaluation of T lymphocytes in synovial fluid, synovium, and peripheral circulation
AU - Kennedy-Stoskopf, Suzanne
AU - Zink, Christine
AU - Narayan, Opendra
N1 - Funding Information:
We thank M. Miyasaka and the Base1 Institute of Immunology for providing the monoclonal antibodies, and D. Sheffer and J. Baker for technical support. SBU-T4 was supplied by M. R. Brandon and M. Gorrell at the University of Melbourne. These studies were supported by Grants NS12127, NS21916, RROO130, and NS07000. S.K.S. was supported by a Special Emphasis Research Career Award KOl RR00017 from the NIH and a Johns Hopkins Institutional Research Grant. C.Z. was supported by a fellowship from the Medical Research Council of Canada.
PY - 1989/8
Y1 - 1989/8
N2 - Sheep and goats develop a chronic, progressive arthritis reminiscent of rheumatoid arthritis, but caused by lentiviruses related to human immunodeficiency virus. The distribution of T lymphocytes in peripheral circulation of two infected sheep with arthritis, one infected sheep with interstitial pneumonia, three asymptomatic sheep, and three uninfected sheep was evaluated. Sheep with clinical disease have depressed ratios of CD4/CD8 lymphocytes in peripheral circulation compared to asymptomatic and uninfected animals. In one sheep, the depressed ratio was due to an absolute increase in CD8-positive lymhocytes. The predominant lymphocyte populations in both synovial fluid and synovium from this animal were also CD8 positive. Macrophages were the other predominant cell population in synovial fluid and were infected with lentivirus. Little cell-free virus was detected in the synovial fluid, although 1 in 400 cells was infected as determined by infectious center assays. Infected cells in the synovial fluid had a reduction in virus gene expression compared to infected cells in peripheral circulation. This reduction in virus gene expression may be due to the presence of interferon-like activity in the synovial fluid.
AB - Sheep and goats develop a chronic, progressive arthritis reminiscent of rheumatoid arthritis, but caused by lentiviruses related to human immunodeficiency virus. The distribution of T lymphocytes in peripheral circulation of two infected sheep with arthritis, one infected sheep with interstitial pneumonia, three asymptomatic sheep, and three uninfected sheep was evaluated. Sheep with clinical disease have depressed ratios of CD4/CD8 lymphocytes in peripheral circulation compared to asymptomatic and uninfected animals. In one sheep, the depressed ratio was due to an absolute increase in CD8-positive lymhocytes. The predominant lymphocyte populations in both synovial fluid and synovium from this animal were also CD8 positive. Macrophages were the other predominant cell population in synovial fluid and were infected with lentivirus. Little cell-free virus was detected in the synovial fluid, although 1 in 400 cells was infected as determined by infectious center assays. Infected cells in the synovial fluid had a reduction in virus gene expression compared to infected cells in peripheral circulation. This reduction in virus gene expression may be due to the presence of interferon-like activity in the synovial fluid.
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U2 - 10.1016/0090-1229(89)90183-9
DO - 10.1016/0090-1229(89)90183-9
M3 - Article
C2 - 2472238
AN - SCOPUS:0024359998
SN - 0090-1229
VL - 52
SP - 323
EP - 330
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 2
ER -