Pathogenesis of hypoxic-ischemic brain injury in a perinatal rodent model

Faye SilVerstein; Karen Buchanan; Michael V. Johnston

doi:10.1016/0304-3940(84)90301-X

Pathogenesis of hypoxic-ischemic brain injury in a perinatal rodent model

Faye SilVerstein, Karen Buchanan, Michael V. Johnston

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Exposure of immature rats to 8% oxygen after unilateral carotid artery ligation (UCL) causes metabolic, neurochemical and histopathological changes in the ipsilateral forebrain that resemble those in human perinatal hypoxic-ischemic encephalopathy. Regional cerebral perfusion in this model was examined by visual analysis of India ink trapped in cerebral vessels and measurement of [¹⁴C]iodoantipyrine ([¹⁴C]IAP) and [³H]flunitrazepam extraction into the brain. UCL alone reduced [¹⁴C]IAP accumulation in the ipsilateral hemisphere by 20% and hypoxia superimposed on UCL progressively reduced ipsilateral hemisphere perfusion by 71% at 2 h. Hypoxia probably injures neurons in this model by causing a critical reduction in cerebral perfusion, an effect which also appears to be important in the human disorder.

Original language	English (US)
Pages (from-to)	271-277
Number of pages	7
Journal	Neuroscience Letters
Volume	49
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(84)90301-X
State	Published - Aug 31 1984
Externally published	Yes

Keywords

[C]iodoantipyrine
cerebral blood flow
hypoxia-ischemia
immature rat

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(84)90301-X

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title = "Pathogenesis of hypoxic-ischemic brain injury in a perinatal rodent model",

abstract = "Exposure of immature rats to 8% oxygen after unilateral carotid artery ligation (UCL) causes metabolic, neurochemical and histopathological changes in the ipsilateral forebrain that resemble those in human perinatal hypoxic-ischemic encephalopathy. Regional cerebral perfusion in this model was examined by visual analysis of India ink trapped in cerebral vessels and measurement of [14C]iodoantipyrine ([14C]IAP) and [3H]flunitrazepam extraction into the brain. UCL alone reduced [14C]IAP accumulation in the ipsilateral hemisphere by 20% and hypoxia superimposed on UCL progressively reduced ipsilateral hemisphere perfusion by 71% at 2 h. Hypoxia probably injures neurons in this model by causing a critical reduction in cerebral perfusion, an effect which also appears to be important in the human disorder.",

keywords = "[C]iodoantipyrine, cerebral blood flow, hypoxia-ischemia, immature rat",

author = "Faye SilVerstein and Karen Buchanan and Johnston, {Michael V.}",

note = "Funding Information: Supported in part by Grant NS R017542 from N.I.H., Grant R326 from United Cerebral Palsy Research and Education Foundation, and T.I.D.A. NS00603 from NINCDS (M.V.J.). F.S.S. has a fellowship from the Medical Research Council of Canada.",

year = "1984",

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doi = "10.1016/0304-3940(84)90301-X",

language = "English (US)",

volume = "49",

pages = "271--277",

journal = "Neuroscience Letters",

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T1 - Pathogenesis of hypoxic-ischemic brain injury in a perinatal rodent model

AU - SilVerstein, Faye

AU - Buchanan, Karen

AU - Johnston, Michael V.

N1 - Funding Information: Supported in part by Grant NS R017542 from N.I.H., Grant R326 from United Cerebral Palsy Research and Education Foundation, and T.I.D.A. NS00603 from NINCDS (M.V.J.). F.S.S. has a fellowship from the Medical Research Council of Canada.

PY - 1984/8/31

Y1 - 1984/8/31

N2 - Exposure of immature rats to 8% oxygen after unilateral carotid artery ligation (UCL) causes metabolic, neurochemical and histopathological changes in the ipsilateral forebrain that resemble those in human perinatal hypoxic-ischemic encephalopathy. Regional cerebral perfusion in this model was examined by visual analysis of India ink trapped in cerebral vessels and measurement of [14C]iodoantipyrine ([14C]IAP) and [3H]flunitrazepam extraction into the brain. UCL alone reduced [14C]IAP accumulation in the ipsilateral hemisphere by 20% and hypoxia superimposed on UCL progressively reduced ipsilateral hemisphere perfusion by 71% at 2 h. Hypoxia probably injures neurons in this model by causing a critical reduction in cerebral perfusion, an effect which also appears to be important in the human disorder.

AB - Exposure of immature rats to 8% oxygen after unilateral carotid artery ligation (UCL) causes metabolic, neurochemical and histopathological changes in the ipsilateral forebrain that resemble those in human perinatal hypoxic-ischemic encephalopathy. Regional cerebral perfusion in this model was examined by visual analysis of India ink trapped in cerebral vessels and measurement of [14C]iodoantipyrine ([14C]IAP) and [3H]flunitrazepam extraction into the brain. UCL alone reduced [14C]IAP accumulation in the ipsilateral hemisphere by 20% and hypoxia superimposed on UCL progressively reduced ipsilateral hemisphere perfusion by 71% at 2 h. Hypoxia probably injures neurons in this model by causing a critical reduction in cerebral perfusion, an effect which also appears to be important in the human disorder.

KW - [C]iodoantipyrine

KW - cerebral blood flow

KW - hypoxia-ischemia

KW - immature rat

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JO - Neuroscience Letters

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ER -

Pathogenesis of hypoxic-ischemic brain injury in a perinatal rodent model

Abstract

Keywords

ASJC Scopus subject areas

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