Pathogenesis of Acute Infection in Rhesus Macaques with a Lymphocyte-Tropic Strain of Simian Immunodeficiency Virus

Dharam P. Sharma, Mark Anderson, M. Christine Zink, Robert J. Adams, Albert D. Donnenberg, Janice E. Clements, Opendra Narayan

Research output: Contribution to journalArticle

Abstract

The simian immunodeficiency virus, SIVmac, causes disease affecting multiple organ systems in macaques similar to human immunodeficiency virus infection in humans. Molecularly cloned SIVmac with a strong lymphocyte tropism was used in pathogenesis experiments to correlate viral cell tropism with disease. In 5 animals, exhaustive analyses on viruses from tissues and identification of infected precursor cells were done at multiple times during infection to ensure the virus had not mutated into a macrophage-tropic variant. Viral replication was measured by infectivity, infectious center assays, and in situ hybridization. Lymphocytes produced most virus in tissues, indicating the virus maintained its cell tropism in vivo. Lymphocytes in bone marrow were latently infected and those in the spleen and lymph nodes were productively infected. The virus failed to replicate in the brain after intracerebral inoculation. SIVma(. that maintained a strong tropism for lymphocytes and a corresponding poor tropism for macrophages can cause persistent infection and AIDS but not other diseases such as primary pneumonia and encephalitis in rhesus macaques.

Original languageEnglish (US)
Pages (from-to)738-746
Number of pages9
JournalJournal of Infectious Diseases
Volume166
Issue number4
DOIs
StatePublished - Oct 1992

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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