Pathogen-pathogen interactions: A comparative study of Escherichia coli interactions with the clinical and environmental isolates of Acanthamoeba

Suk Yul Jung, Selwa Alsam, Kwang Sik Kim, Naveed Ahmed Khan

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we compared the interactions of invasive and non-invasive strains of E. coli with clinical and environmental isolates of Acanthamoeba. The environmental isolate of Acanthamoeba exhibited significantly higher association with E. coli compared with the clinical isolates of Acanthamoeba. The ratio of E. coli per amoebae was more than 8-fold higher in the environmental isolate compared with the clinical isolates of Acanthamoeba. Interestingly, non-pathogenic environmental Acanthamoeba showed uptake and/or survival of the non-invasive E. coli. In contrast, clinical isolates of Acanthamoeba did not support uptake and/or survival of non-invasive E. coli. Using several mutants derived from K1, we demonstrated that outer membrane protein A (OmpA) and lipopolysaccharide (LPS) are crucial bacterial determinants responsible for E. coli K1 interactions and in the intracellular survival of E. coli in Acanthamoeba. The use of Acanthamoeba as a model to study E. coli K1 pathogenesis and to understand bacterial immune evasion strategies is discussed further.

Original languageEnglish (US)
Pages (from-to)2339-2348
Number of pages10
JournalWorld Journal of Microbiology and Biotechnology
Volume24
Issue number10
DOIs
StatePublished - Oct 1 2008

Keywords

  • Acanthamoeba
  • Escherichia coli
  • Invasion
  • Lipopolysaccharide
  • Outer membrane protein A
  • Survival

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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