Pathogen blocking in wolbachia-infected aedes aegypti is not affected by zika and dengue virus co-infection

Eric Caragata, Marcele N. Rocha, Thiago N. Pereira, Simone B. Mansur, Heverton L.C. Dutra, Luciano A. Moreira

Research output: Contribution to journalArticle

Abstract

Background Wolbachia’s ability to restrict arbovirus transmission makes it a promising tool to combat mosquito-transmitted diseases. Wolbachia-infected Aedes aegypti are currently being released in locations such as Brazil, which regularly experience concurrent outbreaks of different arboviruses. A. aegypti can become co-infected with, and transmit multiple arboviruses with one bite, which can complicate patient diagnosis and treatment. Methodology/principle findings Using experimental oral infection of A. aegypti and then RT-qPCR, we examined ZIKV/ DENV-1 and ZIKV/DENV-3 co-infection in Wolbachia-infected A. aegypti and observed that Wolbachia-infected mosquitoes experienced lower prevalence of infection and viral load than wildtype mosquitoes, even with an extra infecting virus. Critically, ZIKV/DENV co-infection had no significant impact on Wolbachia’s ability to reduce viral transmission. Wolbachia infection also strongly altered expression levels of key immune genes Defensin C and Transferrin 1, in a virus-dependent manner. Conclusions/significance Our results suggest that pathogen interference in Wolbachia-infected A. aegypti is not adversely affected by ZIKV/DENV co-infection, which suggests that Wolbachia-infected A. aegypti will likely prove suitable for controlling mosquito-borne diseases in environments with complex patterns of arbovirus transmission.

Original languageEnglish (US)
Article numbere0007443
JournalPLoS neglected tropical diseases
Volume13
Issue number5
DOIs
StatePublished - May 1 2019

Fingerprint

Wolbachia
Dengue Virus
Aedes
Virus Diseases
Coinfection
Arboviruses
Culicidae
Infection
Viruses
Defensins
Bites and Stings
Transferrin
Viral Load
Disease Outbreaks
Brazil
Zika Virus
Genes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Pathogen blocking in wolbachia-infected aedes aegypti is not affected by zika and dengue virus co-infection. / Caragata, Eric; Rocha, Marcele N.; Pereira, Thiago N.; Mansur, Simone B.; Dutra, Heverton L.C.; Moreira, Luciano A.

In: PLoS neglected tropical diseases, Vol. 13, No. 5, e0007443, 01.05.2019.

Research output: Contribution to journalArticle

Caragata, Eric ; Rocha, Marcele N. ; Pereira, Thiago N. ; Mansur, Simone B. ; Dutra, Heverton L.C. ; Moreira, Luciano A. / Pathogen blocking in wolbachia-infected aedes aegypti is not affected by zika and dengue virus co-infection. In: PLoS neglected tropical diseases. 2019 ; Vol. 13, No. 5.
@article{a26b49b4a2a84d23805aee3102b39035,
title = "Pathogen blocking in wolbachia-infected aedes aegypti is not affected by zika and dengue virus co-infection",
abstract = "Background Wolbachia’s ability to restrict arbovirus transmission makes it a promising tool to combat mosquito-transmitted diseases. Wolbachia-infected Aedes aegypti are currently being released in locations such as Brazil, which regularly experience concurrent outbreaks of different arboviruses. A. aegypti can become co-infected with, and transmit multiple arboviruses with one bite, which can complicate patient diagnosis and treatment. Methodology/principle findings Using experimental oral infection of A. aegypti and then RT-qPCR, we examined ZIKV/ DENV-1 and ZIKV/DENV-3 co-infection in Wolbachia-infected A. aegypti and observed that Wolbachia-infected mosquitoes experienced lower prevalence of infection and viral load than wildtype mosquitoes, even with an extra infecting virus. Critically, ZIKV/DENV co-infection had no significant impact on Wolbachia’s ability to reduce viral transmission. Wolbachia infection also strongly altered expression levels of key immune genes Defensin C and Transferrin 1, in a virus-dependent manner. Conclusions/significance Our results suggest that pathogen interference in Wolbachia-infected A. aegypti is not adversely affected by ZIKV/DENV co-infection, which suggests that Wolbachia-infected A. aegypti will likely prove suitable for controlling mosquito-borne diseases in environments with complex patterns of arbovirus transmission.",
author = "Eric Caragata and Rocha, {Marcele N.} and Pereira, {Thiago N.} and Mansur, {Simone B.} and Dutra, {Heverton L.C.} and Moreira, {Luciano A.}",
year = "2019",
month = "5",
day = "1",
doi = "10.1371/journal.pntd.0007443",
language = "English (US)",
volume = "13",
journal = "PLoS Neglected Tropical Diseases",
issn = "1935-2727",
publisher = "Public Library of Science",
number = "5",

}

TY - JOUR

T1 - Pathogen blocking in wolbachia-infected aedes aegypti is not affected by zika and dengue virus co-infection

AU - Caragata, Eric

AU - Rocha, Marcele N.

AU - Pereira, Thiago N.

AU - Mansur, Simone B.

AU - Dutra, Heverton L.C.

AU - Moreira, Luciano A.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Background Wolbachia’s ability to restrict arbovirus transmission makes it a promising tool to combat mosquito-transmitted diseases. Wolbachia-infected Aedes aegypti are currently being released in locations such as Brazil, which regularly experience concurrent outbreaks of different arboviruses. A. aegypti can become co-infected with, and transmit multiple arboviruses with one bite, which can complicate patient diagnosis and treatment. Methodology/principle findings Using experimental oral infection of A. aegypti and then RT-qPCR, we examined ZIKV/ DENV-1 and ZIKV/DENV-3 co-infection in Wolbachia-infected A. aegypti and observed that Wolbachia-infected mosquitoes experienced lower prevalence of infection and viral load than wildtype mosquitoes, even with an extra infecting virus. Critically, ZIKV/DENV co-infection had no significant impact on Wolbachia’s ability to reduce viral transmission. Wolbachia infection also strongly altered expression levels of key immune genes Defensin C and Transferrin 1, in a virus-dependent manner. Conclusions/significance Our results suggest that pathogen interference in Wolbachia-infected A. aegypti is not adversely affected by ZIKV/DENV co-infection, which suggests that Wolbachia-infected A. aegypti will likely prove suitable for controlling mosquito-borne diseases in environments with complex patterns of arbovirus transmission.

AB - Background Wolbachia’s ability to restrict arbovirus transmission makes it a promising tool to combat mosquito-transmitted diseases. Wolbachia-infected Aedes aegypti are currently being released in locations such as Brazil, which regularly experience concurrent outbreaks of different arboviruses. A. aegypti can become co-infected with, and transmit multiple arboviruses with one bite, which can complicate patient diagnosis and treatment. Methodology/principle findings Using experimental oral infection of A. aegypti and then RT-qPCR, we examined ZIKV/ DENV-1 and ZIKV/DENV-3 co-infection in Wolbachia-infected A. aegypti and observed that Wolbachia-infected mosquitoes experienced lower prevalence of infection and viral load than wildtype mosquitoes, even with an extra infecting virus. Critically, ZIKV/DENV co-infection had no significant impact on Wolbachia’s ability to reduce viral transmission. Wolbachia infection also strongly altered expression levels of key immune genes Defensin C and Transferrin 1, in a virus-dependent manner. Conclusions/significance Our results suggest that pathogen interference in Wolbachia-infected A. aegypti is not adversely affected by ZIKV/DENV co-infection, which suggests that Wolbachia-infected A. aegypti will likely prove suitable for controlling mosquito-borne diseases in environments with complex patterns of arbovirus transmission.

UR - http://www.scopus.com/inward/record.url?scp=85066965694&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85066965694&partnerID=8YFLogxK

U2 - 10.1371/journal.pntd.0007443

DO - 10.1371/journal.pntd.0007443

M3 - Article

VL - 13

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

SN - 1935-2727

IS - 5

M1 - e0007443

ER -