TY - JOUR
T1 - Patch clamp study of mouse glomus cells using a whole carotid body
AU - Yamaguchi, Shigeki
AU - Lande, Boris
AU - Kitajima, Toshimitsu
AU - Hori, Yuichi
AU - Shirahata, Machiko
N1 - Funding Information:
This work was supported by AHA0255358N, NHLBI HL61596, NHLBI HL66324 (USA) and Ministry of Education 1579083 (Japan).
PY - 2004/3/4
Y1 - 2004/3/4
N2 - Some electrophysiological characteristics of mouse glomus cells (DBA/2J strain) were investigated using an undissociated carotid body. The carotid body with major carotid arteries was placed in a recording chamber, and glomus cells were visualized with a water immersion lens combined with an infrared differential interference video camera. Patch clamp experiments revealed that voltage-gated outward current, but not inward current, was easily observed in glomus cells. Pharmacological experiments and the kinetics of the current suggest that outward current is via delayed rectifier, A type, and large conductance calcium-activated K channels. Furthermore, K current was reversibly attenuated by mild hypoxia. The results suggest electrophysiological similarities of glomus cells among the cat, the rat, and the DBA/2J mouse. The method appears useful for physiological experiments.
AB - Some electrophysiological characteristics of mouse glomus cells (DBA/2J strain) were investigated using an undissociated carotid body. The carotid body with major carotid arteries was placed in a recording chamber, and glomus cells were visualized with a water immersion lens combined with an infrared differential interference video camera. Patch clamp experiments revealed that voltage-gated outward current, but not inward current, was easily observed in glomus cells. Pharmacological experiments and the kinetics of the current suggest that outward current is via delayed rectifier, A type, and large conductance calcium-activated K channels. Furthermore, K current was reversibly attenuated by mild hypoxia. The results suggest electrophysiological similarities of glomus cells among the cat, the rat, and the DBA/2J mouse. The method appears useful for physiological experiments.
KW - Chemoreceptor
KW - DBA/2J strain
KW - Hypoxia
KW - K channel
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U2 - 10.1016/j.neulet.2003.10.062
DO - 10.1016/j.neulet.2003.10.062
M3 - Article
C2 - 15036598
AN - SCOPUS:1242269802
VL - 357
SP - 155
EP - 157
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -