Patch clamp study of mouse glomus cells using a whole carotid body

Shigeki Yamaguchi; Boris Lande; Toshimitsu Kitajima; Yuichi Hori; Machiko Shirahata

doi:10.1016/j.neulet.2003.10.062

Patch clamp study of mouse glomus cells using a whole carotid body

Shigeki Yamaguchi, Boris Lande, Toshimitsu Kitajima, Yuichi Hori, Machiko Shirahata

Bloomberg School of Public Health

Research output: Contribution to journal › Article

Abstract

Some electrophysiological characteristics of mouse glomus cells (DBA/2J strain) were investigated using an undissociated carotid body. The carotid body with major carotid arteries was placed in a recording chamber, and glomus cells were visualized with a water immersion lens combined with an infrared differential interference video camera. Patch clamp experiments revealed that voltage-gated outward current, but not inward current, was easily observed in glomus cells. Pharmacological experiments and the kinetics of the current suggest that outward current is via delayed rectifier, A type, and large conductance calcium-activated K channels. Furthermore, K current was reversibly attenuated by mild hypoxia. The results suggest electrophysiological similarities of glomus cells among the cat, the rat, and the DBA/2J mouse. The method appears useful for physiological experiments.

Original language	English (US)
Pages (from-to)	155-157
Number of pages	3
Journal	Neuroscience Letters
Volume	357
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2003.10.062
State	Published - Mar 4 2004

Keywords

Chemoreceptor
DBA/2J strain
Hypoxia
K channel

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/j.neulet.2003.10.062

Fingerprint Dive into the research topics of 'Patch clamp study of mouse glomus cells using a whole carotid body'. Together they form a unique fingerprint.

Cite this

Yamaguchi, S., Lande, B., Kitajima, T., Hori, Y., & Shirahata, M. (2004). Patch clamp study of mouse glomus cells using a whole carotid body. Neuroscience Letters, 357(2), 155-157. https://doi.org/10.1016/j.neulet.2003.10.062

@article{d160a737aeb54c2296c815f9c7f16041,

title = "Patch clamp study of mouse glomus cells using a whole carotid body",

abstract = "Some electrophysiological characteristics of mouse glomus cells (DBA/2J strain) were investigated using an undissociated carotid body. The carotid body with major carotid arteries was placed in a recording chamber, and glomus cells were visualized with a water immersion lens combined with an infrared differential interference video camera. Patch clamp experiments revealed that voltage-gated outward current, but not inward current, was easily observed in glomus cells. Pharmacological experiments and the kinetics of the current suggest that outward current is via delayed rectifier, A type, and large conductance calcium-activated K channels. Furthermore, K current was reversibly attenuated by mild hypoxia. The results suggest electrophysiological similarities of glomus cells among the cat, the rat, and the DBA/2J mouse. The method appears useful for physiological experiments.",

keywords = "Chemoreceptor, DBA/2J strain, Hypoxia, K channel",

author = "Shigeki Yamaguchi and Boris Lande and Toshimitsu Kitajima and Yuichi Hori and Machiko Shirahata",

year = "2004",

month = mar,

day = "4",

doi = "10.1016/j.neulet.2003.10.062",

language = "English (US)",

volume = "357",

pages = "155--157",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Patch clamp study of mouse glomus cells using a whole carotid body

AU - Yamaguchi, Shigeki

AU - Lande, Boris

AU - Kitajima, Toshimitsu

AU - Hori, Yuichi

AU - Shirahata, Machiko

PY - 2004/3/4

Y1 - 2004/3/4

N2 - Some electrophysiological characteristics of mouse glomus cells (DBA/2J strain) were investigated using an undissociated carotid body. The carotid body with major carotid arteries was placed in a recording chamber, and glomus cells were visualized with a water immersion lens combined with an infrared differential interference video camera. Patch clamp experiments revealed that voltage-gated outward current, but not inward current, was easily observed in glomus cells. Pharmacological experiments and the kinetics of the current suggest that outward current is via delayed rectifier, A type, and large conductance calcium-activated K channels. Furthermore, K current was reversibly attenuated by mild hypoxia. The results suggest electrophysiological similarities of glomus cells among the cat, the rat, and the DBA/2J mouse. The method appears useful for physiological experiments.

AB - Some electrophysiological characteristics of mouse glomus cells (DBA/2J strain) were investigated using an undissociated carotid body. The carotid body with major carotid arteries was placed in a recording chamber, and glomus cells were visualized with a water immersion lens combined with an infrared differential interference video camera. Patch clamp experiments revealed that voltage-gated outward current, but not inward current, was easily observed in glomus cells. Pharmacological experiments and the kinetics of the current suggest that outward current is via delayed rectifier, A type, and large conductance calcium-activated K channels. Furthermore, K current was reversibly attenuated by mild hypoxia. The results suggest electrophysiological similarities of glomus cells among the cat, the rat, and the DBA/2J mouse. The method appears useful for physiological experiments.

KW - Chemoreceptor

KW - DBA/2J strain

KW - Hypoxia

KW - K channel

UR - http://www.scopus.com/inward/record.url?scp=1242269802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1242269802&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2003.10.062

DO - 10.1016/j.neulet.2003.10.062

M3 - Article

C2 - 15036598

AN - SCOPUS:1242269802

VL - 357

SP - 155

EP - 157

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2

ER -