Patch clamp study of mouse glomus cells using a whole carotid body

Shigeki Yamaguchi, Boris Lande, Toshimitsu Kitajima, Yuichi Hori, Machiko Shirahata

Research output: Contribution to journalArticle

Abstract

Some electrophysiological characteristics of mouse glomus cells (DBA/2J strain) were investigated using an undissociated carotid body. The carotid body with major carotid arteries was placed in a recording chamber, and glomus cells were visualized with a water immersion lens combined with an infrared differential interference video camera. Patch clamp experiments revealed that voltage-gated outward current, but not inward current, was easily observed in glomus cells. Pharmacological experiments and the kinetics of the current suggest that outward current is via delayed rectifier, A type, and large conductance calcium-activated K channels. Furthermore, K current was reversibly attenuated by mild hypoxia. The results suggest electrophysiological similarities of glomus cells among the cat, the rat, and the DBA/2J mouse. The method appears useful for physiological experiments.

Original languageEnglish (US)
Pages (from-to)155-157
Number of pages3
JournalNeuroscience Letters
Volume357
Issue number2
DOIs
StatePublished - Mar 4 2004

Keywords

  • Chemoreceptor
  • DBA/2J strain
  • Hypoxia
  • K channel

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Yamaguchi, S., Lande, B., Kitajima, T., Hori, Y., & Shirahata, M. (2004). Patch clamp study of mouse glomus cells using a whole carotid body. Neuroscience Letters, 357(2), 155-157. https://doi.org/10.1016/j.neulet.2003.10.062