Passive transfer of modest titers of potent and broadly neutralizing anti-HIV monoclonal antibodies block SHIV infection in macaques

Masashi Shingai, Olivia K. Donau, Ronald J. Plishka, Alicia Buckler-White, John R. Mascola, Gary J. Nabel, Martha C. Nason, David Montefiori, Brian Moldt, Pascal Poignard, Ron Diskin, Pamela J. Bjorkman, Michael A. Eckhaus, Florian Klein, Hugo Mouquet, Julio Cesar Cetrulo Lorenzi, Anna Gazumyan, Dennis R. Burton, Michel C. Nussenzweig, Malcolm A. MartinYoshiaki Nishimura

Research output: Contribution to journalArticle

Abstract

It is widely appreciated that effective human vaccines directed against viral pathogens elicit neutralizing antibodies (NAbs). The passive transfer of anti-HIV-1 NAbs conferring sterilizing immunity to macaques has been used to determine the plasma neutralization titers, which must be present at the time of exposure, to prevent acquisition of SIV/HIV chimeric virus (SHIV) infections. We administered five recently isolated potent and broadly acting anti-HIV neutralizing monoclonal antibodies (mAbs) to rhesus macaques and challenged them intrarectally 24 h later with either of two different R5-tropic SHIVs. By combining the results obtained from 60 challenged animals, we determined that the protective neutralization titer in plasma preventing virus infection in 50% of the exposed monkeys was relatively modest (∼1:100) and potentially achievable by vaccination.

Original languageEnglish (US)
Pages (from-to)2061-2074
Number of pages14
JournalJournal of Experimental Medicine
Volume211
Issue number10
DOIs
StatePublished - 2014
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

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    Shingai, M., Donau, O. K., Plishka, R. J., Buckler-White, A., Mascola, J. R., Nabel, G. J., Nason, M. C., Montefiori, D., Moldt, B., Poignard, P., Diskin, R., Bjorkman, P. J., Eckhaus, M. A., Klein, F., Mouquet, H., Lorenzi, J. C. C., Gazumyan, A., Burton, D. R., Nussenzweig, M. C., ... Nishimura, Y. (2014). Passive transfer of modest titers of potent and broadly neutralizing anti-HIV monoclonal antibodies block SHIV infection in macaques. Journal of Experimental Medicine, 211(10), 2061-2074. https://doi.org/10.1084/jem.20132494