pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland

Partha Seshaiah, Bret Miller, Monn Monn Myat, Deborah J. Andrew

Research output: Contribution to journalArticle

Abstract

From a screen for genes expressed and required in the Drosophila salivary gland, we identified pasilla (ps), which encodes a set of proteins most similar to human Nova-1 and Nova-2. Nova-1 and Nova-2 are nuclear RNA-binding proteins normally expressed in the CNS where they directly regulate splicing. In patients suffering from paraneoplastic opsoclonus myoclonus ataxia (POMA), Nova-1 and Nova-2 proteins are present as auto-antigens. Consistent with a role in splicing, PS is localized to nuclear puncta. The salivary glands of ps mutants internalize normally and maintain epithelial polarity. However, the mutant salivary glands develop irregularities in overall morphology and have defects in apical secretion. The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients.

Original languageEnglish (US)
Pages (from-to)309-322
Number of pages14
JournalDevelopmental biology
Volume239
Issue number2
DOIs
StatePublished - Nov 15 2001

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Keywords

  • Drosophila
  • KH-domain
  • Morphogenesis
  • Nova-1
  • Nova-2
  • POMA
  • RNA-binding protein
  • Salivary gland
  • Secretion
  • Splicing

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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