pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland

Partha Seshaiah, Bret Miller, Monn Monn Myat, Deborah J Andrew

Research output: Contribution to journalArticle

Abstract

From a screen for genes expressed and required in the Drosophila salivary gland, we identified pasilla (ps), which encodes a set of proteins most similar to human Nova-1 and Nova-2. Nova-1 and Nova-2 are nuclear RNA-binding proteins normally expressed in the CNS where they directly regulate splicing. In patients suffering from paraneoplastic opsoclonus myoclonus ataxia (POMA), Nova-1 and Nova-2 proteins are present as auto-antigens. Consistent with a role in splicing, PS is localized to nuclear puncta. The salivary glands of ps mutants internalize normally and maintain epithelial polarity. However, the mutant salivary glands develop irregularities in overall morphology and have defects in apical secretion. The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients.

Original languageEnglish (US)
Pages (from-to)309-322
Number of pages14
JournalDevelopmental Biology
Volume239
Issue number2
DOIs
StatePublished - Nov 15 2001

Fingerprint

Opsoclonus-Myoclonus Syndrome
Salivary Glands
Drosophila
Nuclear RNA
RNA-Binding Proteins
Nuclear Proteins
Antigens
Genes
Nova antigen
Proteins

Keywords

  • Drosophila
  • KH-domain
  • Morphogenesis
  • Nova-1
  • Nova-2
  • POMA
  • RNA-binding protein
  • Salivary gland
  • Secretion
  • Splicing

ASJC Scopus subject areas

  • Developmental Biology

Cite this

pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland. / Seshaiah, Partha; Miller, Bret; Myat, Monn Monn; Andrew, Deborah J.

In: Developmental Biology, Vol. 239, No. 2, 15.11.2001, p. 309-322.

Research output: Contribution to journalArticle

@article{a6f85dede2e249cc8415c58becb193d8,
title = "pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland",
abstract = "From a screen for genes expressed and required in the Drosophila salivary gland, we identified pasilla (ps), which encodes a set of proteins most similar to human Nova-1 and Nova-2. Nova-1 and Nova-2 are nuclear RNA-binding proteins normally expressed in the CNS where they directly regulate splicing. In patients suffering from paraneoplastic opsoclonus myoclonus ataxia (POMA), Nova-1 and Nova-2 proteins are present as auto-antigens. Consistent with a role in splicing, PS is localized to nuclear puncta. The salivary glands of ps mutants internalize normally and maintain epithelial polarity. However, the mutant salivary glands develop irregularities in overall morphology and have defects in apical secretion. The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients.",
keywords = "Drosophila, KH-domain, Morphogenesis, Nova-1, Nova-2, POMA, RNA-binding protein, Salivary gland, Secretion, Splicing",
author = "Partha Seshaiah and Bret Miller and Myat, {Monn Monn} and Andrew, {Deborah J}",
year = "2001",
month = "11",
day = "15",
doi = "10.1006/dbio.2001.0429",
language = "English (US)",
volume = "239",
pages = "309--322",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland

AU - Seshaiah, Partha

AU - Miller, Bret

AU - Myat, Monn Monn

AU - Andrew, Deborah J

PY - 2001/11/15

Y1 - 2001/11/15

N2 - From a screen for genes expressed and required in the Drosophila salivary gland, we identified pasilla (ps), which encodes a set of proteins most similar to human Nova-1 and Nova-2. Nova-1 and Nova-2 are nuclear RNA-binding proteins normally expressed in the CNS where they directly regulate splicing. In patients suffering from paraneoplastic opsoclonus myoclonus ataxia (POMA), Nova-1 and Nova-2 proteins are present as auto-antigens. Consistent with a role in splicing, PS is localized to nuclear puncta. The salivary glands of ps mutants internalize normally and maintain epithelial polarity. However, the mutant salivary glands develop irregularities in overall morphology and have defects in apical secretion. The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients.

AB - From a screen for genes expressed and required in the Drosophila salivary gland, we identified pasilla (ps), which encodes a set of proteins most similar to human Nova-1 and Nova-2. Nova-1 and Nova-2 are nuclear RNA-binding proteins normally expressed in the CNS where they directly regulate splicing. In patients suffering from paraneoplastic opsoclonus myoclonus ataxia (POMA), Nova-1 and Nova-2 proteins are present as auto-antigens. Consistent with a role in splicing, PS is localized to nuclear puncta. The salivary glands of ps mutants internalize normally and maintain epithelial polarity. However, the mutant salivary glands develop irregularities in overall morphology and have defects in apical secretion. The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients.

KW - Drosophila

KW - KH-domain

KW - Morphogenesis

KW - Nova-1

KW - Nova-2

KW - POMA

KW - RNA-binding protein

KW - Salivary gland

KW - Secretion

KW - Splicing

UR - http://www.scopus.com/inward/record.url?scp=0035891190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035891190&partnerID=8YFLogxK

U2 - 10.1006/dbio.2001.0429

DO - 10.1006/dbio.2001.0429

M3 - Article

C2 - 11784037

AN - SCOPUS:0035891190

VL - 239

SP - 309

EP - 322

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -