PASG, an SNF2 family member essential for proliferation of murine lymphocytes and altered in leukemia, is expressed in the proliferatfve compartments of human myeloid and lymphoid progenitors

Regina Wieland, Eric H. Raabe, Robert J. Arceci

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Abstract

SNF2 factorsare nucleosome remodeling ATPases that are involved ingene regulation, DNA repair, and recombinavion. "We recently identified a novel member of this family, PASO, as an mRNA that is altered in leukemia and is associated with cellular proliferation in hematopoietic and non-hematopoietic cells in vitro [Blood 94:594a, 1999; Cancer Res 60:3612, 2000]. Based on our findings in vitro, we hypothesized that PASG expression in humans would correlate with known proliferative populations of lymphohematopoietic precursors. To test this hypothesis, we used multiparameter flow cytometry to sort human thymocytes and bone marrow cells into distinct differentiative populations followed by semi-quantitative PCR to determine the expression level of PASG. In thymus, we found that PASG expression was highest in proliferative precursors. High levels of PASG expression were observed in the primitive proliferative CD 3(low), CD34 +, CD25- and CD 3(low), CD34-, CD25- populations, but more differentiated CD 3(high), CD34-, CD25- cells showed significantly lower levels of PASG expression. Fully mature resting peripheral T cells showed very little PASG expression. In cells sorted from bone marrow, CD 19+ B cell precursors were positive for PASG. CD 34+/CD 38+ early myeloid precursors showed relatively high levels of PASG expression, but more differentiated CD 33+ myeloid precursors showed significant downregulation of PASG expression, and fully differentiated granulocytes showed little to no PASG expression. These results demonstrate that PASG expression is highest in early proliferating compartments of thymic and myeloid lineages and is downregulated in less proliferative and more differentiated populations. The data further suggest that PASG may play a role in the expansion and/or lineage commitment of human lymphoid and myeloid precursors.

Original languageEnglish (US)
Pages (from-to)129b
JournalBlood
Volume96
Issue number11 PART II
StatePublished - Dec 1 2000

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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