Participation of postsynaptic PKC in cerebellar long-term depression in culture

David J. Linden, John A. Connor

Research output: Contribution to journalArticle

Abstract

Long-term depression (LTD) in the intact cerebellum is a decrease in the efficacy of the parallel fiber-Purkinje neuron synapse induced by coactivation of climbing fiber and parallel fiber inputs. In cultured Purkinje neurons, a similar depression can be induced by iontophoretic glutamate pulses and Purkinje neuron depolarization. This form of LTD is expressed as a depression of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA)-mediated current, and its induction is dependent on activation of metabotropic quisqualate receptors. The effect of inhibitors of protein kinase C (PKC) on LTD induction was studied. Inhibitors of PKC blocked LTD induction, while phorbol-12,13-diacetate (PDA), a PKC activator, mimicked LTD. These results suggest that PKC activation is necessary for the induction of cerebellar LTD.

Original languageEnglish (US)
Pages (from-to)1656-1659
Number of pages4
JournalScience
Volume254
Issue number5038
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

ASJC Scopus subject areas

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