Partial trisomies in two spontaneously arising long-lived human keratinocyte lines

J. D. Weaver, G. Stetten, J. W. Littlefield

Research output: Contribution to journalArticlepeer-review

Abstract

During experiments concerning the introduction of oncogenes into normal human keratinocytes, we observed long-lived colonies arising spontaneously at the same low frequency in control cultures as in those transfected with Ha-rasEJ or activated c-myc or both. Two of these were karyotyped early in their life span and showed additional chromosomal material on the short arm of chromosome 9 in one case and of chromosome 18 in the other, whereas the parental cells had a normal karyotype. This indicates the presence of a partial trisomy in each line, although the origin of the extra chromosomal material is not known. A similarly long-lived human keratinocyte line containing an isochromosome of the long arm of chromosome 8 has been described elsewhere. Together these results suggest that the spontaneous occurrence of long-lived lines is more common in human keratinocytes than in fibroblasts and that a triple dose of one or more genes may be the initial event in this process.

Original languageEnglish (US)
Pages (from-to)670-675
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume27
Issue number8
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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