Partial Purification and Characterization of Borna Disease Virions Released from Infected Neuroblastoma Cells

J. M. Pyper, J. E. Clements

Research output: Contribution to journalArticlepeer-review

Abstract

Borna disease is a rare but severe neurological disease of horses and sheep. Borna disease virus (BDV) has not been fully characterized because cell-free virus has not been isolated. Homogenates of infected brain are infectious both for animals and for some cell lines in culture. We report here the partial purification and characterization of cell-free BDV from the tissue culture supernatant of infected human neuroblastoma SKNSH cells. A single negative strand 10-kb RNA was detected in purified virions. Immunoprecipitation analysis of the BDV proteins in purified virions shows the presence of the 60-, 38-, 24-, and 14-kDa proteins previously identified as BDV-specific proteins in infected cells.

Original languageEnglish (US)
Article number71304
Pages (from-to)380-382
Number of pages3
JournalVirology
Volume201
Issue number2
DOIs
StatePublished - Jun 1994

ASJC Scopus subject areas

  • Virology

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