Partial mapping and sequencing of a fish iridovirus genome reveals genes homologous to the frog virus 3 p31, p40 and human eIF2α

Yan Xing Yu, Monique Béarzotti, Patrice Vende, Winfried Ahne, Michel Brémont

Research output: Contribution to journalArticle

Abstract

Iridovirus-like pathogens have been recognized as a cause of serious systemic diseases among feral, cultured and ornamental fish in the recent years. Mortalities of fish due to systemic iridovirus infection reaching 30-100% were observed in Europe, Australia, Japan and Thailand. Up to now, the molecular biology of these important pathogens has been poorly documented. To get better insights on the genomic organization of these piscine iridoviruses, we have constructed a cosmid viral DNA library from the epizootic hematopoietic necrosis virus (EHNV). Two recombinant cosmids (Cos7 and Cos12) have been selected for systematic sequencing. Cos7 and 12 are localized side by side along the genome and cover the 2/3 part of the total EHNV genome which has been estimated to be ~101.47 kb in length. Thirty five kilobase pairs (kbps) from Cos7 and 10 kbps from Cos12 have been determined. Sequence analysis revealed open reading frames (ORF) sharing homologies with sequences from the Frog virus 3 such as the p31 and p40 proteins. Among the others identified ORFs, some of them presented homologies with known protein sequences, such as the human eIF2α protein, and some did not show any significant homologies with sequences available in the databases. But, none were related to Lymphocystis virus, a member of the Iridoviridae family, for which the full genome nucleotide sequence has been determined. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)53-63
Number of pages11
JournalVirus Research
Volume63
Issue number1-2
DOIs
StatePublished - Sep 1999
Externally publishedYes

Fingerprint

Ranavirus
Iridovirus
Cosmids
Fishes
Genome
Sequence Homology
Viruses
Open Reading Frames
Iridoviridae
Necrosis
Genes
Proteins
Viral DNA
Thailand
Gene Library
Sequence Analysis
Molecular Biology
Japan
Databases
Mortality

Keywords

  • Cosmid library
  • EHNV
  • Gene sequencing
  • Iridovirus

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Partial mapping and sequencing of a fish iridovirus genome reveals genes homologous to the frog virus 3 p31, p40 and human eIF2α. / Yu, Yan Xing; Béarzotti, Monique; Vende, Patrice; Ahne, Winfried; Brémont, Michel.

In: Virus Research, Vol. 63, No. 1-2, 09.1999, p. 53-63.

Research output: Contribution to journalArticle

Yu, Yan Xing ; Béarzotti, Monique ; Vende, Patrice ; Ahne, Winfried ; Brémont, Michel. / Partial mapping and sequencing of a fish iridovirus genome reveals genes homologous to the frog virus 3 p31, p40 and human eIF2α. In: Virus Research. 1999 ; Vol. 63, No. 1-2. pp. 53-63.
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