Partial depletion of CREB-binding protein reduces life expectancy in a mouse model of Huntington disease

Alexandra M. Klevytska, Andrew T N Tebbenkamp, Alena Savonenko, David R. Borchelt

Research output: Contribution to journalArticle

Abstract

Previous studies have reported that mutant huntingtin (htt) interferes with cyclic AMP response element binding protein binding protein (CBP)-mediated transcription, possibly by inhibiting the acetylation of histones. In Drosophila models that express fragments of mutant htt, histone deacetylase inhibitors reverse deficits in histone acetylation, rescue photoreceptor degeneration, and prolong their survival. These compounds also improve motor deficits in a transgenic mouse model of Huntington disease (HD). To determine whether endogenous CBP depletion contributes to HD pathogenesis, we crossed HD-N171-82Q transgenic mice with mice harboring a disrupted CBP gene and produced mice with partial (50%) depletion of CBP. This reduction of CBP levels decreased the life expectancy of the HD-N171-82Q Line 6 mouse model. The loss of CBP had no obvious impact on the severity of motor impairment, degeneration of the striatum, mutant htt inclusion formation, or global levels of acetylated histones H3 or H4 in brain. In cell models, we confirmed that mutant htt inclusions recruit human CBP but found no evidence for interactions between soluble forms of mutant htt and CBP. Although we identified no neurological explanation for the decreased life expectancy of HD-N171-82Q mice with partial depletion of CBP, the data are consistent with the notion that CBP function mitigates mutant htt toxicity by a currently unidentified mechanism.

Original languageEnglish (US)
Pages (from-to)396-404
Number of pages9
JournalJournal of Neuropathology and Experimental Neurology
Volume69
Issue number4
DOIs
StatePublished - Apr 2010

Fingerprint

CREB-Binding Protein
Huntington Disease
Life Expectancy
Histones
Acetylation
Transgenic Mice
Cyclic AMP Response Element-Binding Protein
Histone Deacetylase Inhibitors
Protein Binding
Drosophila
Carrier Proteins
Survival
Brain
Genes

Keywords

  • CREB binding protein
  • Huntingtin
  • Knockout mice
  • Neurodegeneration
  • Transgenic mice

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Partial depletion of CREB-binding protein reduces life expectancy in a mouse model of Huntington disease. / Klevytska, Alexandra M.; Tebbenkamp, Andrew T N; Savonenko, Alena; Borchelt, David R.

In: Journal of Neuropathology and Experimental Neurology, Vol. 69, No. 4, 04.2010, p. 396-404.

Research output: Contribution to journalArticle

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