Partial denaturation of Haemophilus influenzae DNA by alkali

Alan D. Cooper, P. C. Huang

Research output: Contribution to journalArticlepeer-review

Abstract

Suboptimal condition of alkaline denaturation was used to partially denature Haemophilus influenzae DNA and fractions from these DNA molecules possessing various degrees of strandedness were resolved by hydroxyapatite column chromatography. Exonuclease I sensitivity as well as transforming activity were used to elucidate the secondary structure of these fractions. Fractions supposedly corresponding to the single- and double-stranded DNA were shown to be different from the completely denatured and native DNA, respectively.

Original languageEnglish (US)
Pages (from-to)180-185
Number of pages6
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume238
Issue number2
DOIs
StatePublished - May 13 1971

ASJC Scopus subject areas

  • Medicine(all)

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