Part I. Bcl-2 and Bcl-X(L) limit apoptosis upon infection with alphavirus vectors

Alison J. Mastrangelo, J. Marie Hardwick, Françoise Bex, Michael J. Betenbaugh

Research output: Contribution to journalArticlepeer-review

Abstract

The Sindbis virus (SV) and Semliki forest virus (SFV) were used to infect two commercially relevant cell lines, baby hamster kidney (BHK) and Chinese hamster ovary (CHO). The first was a fully competent SV derivative carrying the gene for chloramphenicol acetyltransferase, while the second was a replication deficient SFV construct containing the human interleukin-12 p35 and p40 genes. Sinde infection with these vectors induced apoptosis in both cell lines, it was determined that the anti-apoptosis genes limit the cell death associated with these virus constructs.

Original languageEnglish (US)
Pages (from-to)544-554
Number of pages11
JournalBiotechnology and bioengineering
Volume67
Issue number5
DOIs
StatePublished - Mar 5 2000

Keywords

  • Apoptosis
  • Chloramphenicol acetyltransferase
  • Interleukin-12
  • Mammalian cell culture
  • Semliki Forest virus
  • Sindbis virus

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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