Paraquat toxicity in a mouse embryonic stem cell model

Venu Perla, Nancy A Perrin, Anne R. Greenlee

Research output: Contribution to journalArticle

Abstract

The objective of this in vitro study was to use a mouse embryonic stem (mES) cell model to better understand pesticide injury that may adversely affect early pregnancy and to evaluate an antioxidant intervention. Undifferentiated D3 mES cells were incubated 24 h with control, reference dose (RfD), no observed effect level (NOEL), or lowest observed effect level (LOEL) of paraquat, a commonly used, toxic agricultural herbicide. Pesticide effects were evaluated at 0 and 24 h using assays for cell proliferation, total reactive oxygen species (ROS), viability, and alkaline phosphatase activity. Compared to 0 h, cell proliferation increased significantly in the 24 h control treatment and was stalled in all paraquat dilutions tested. ROS production and percent necrotic and apoptotic cells were significantly increased at all paraquat concentrations examined. Alkaline phosphatase activity suggested that cells remained undifferentiated during the study period. Experiments with ascorbic acid suggested that pesticide effects on cell viability and ROS production were minimized by the recommended daily allowance (RDA) of vitamin C. Data suggest pesticide-induced injury can occur very early in development and at concentrations predicted without health consequences. Mouse ES cells may provide a useful in vitro model for rapidly screening developmental toxicants and protective interventions.

Original languageEnglish (US)
Pages (from-to)515-524
Number of pages10
JournalToxicology in Vitro
Volume22
Issue number2
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Paraquat
Pesticide effects
Stem cells
Pesticides
Toxicity
Reactive Oxygen Species
Cell proliferation
Ascorbic Acid
Alkaline Phosphatase
Cells
Poisons
Cell Proliferation
Herbicides
No-Observed-Adverse-Effect Level
Recommended Dietary Allowances
Dilution
Assays
Screening
Wounds and Injuries
Antioxidants

Keywords

  • Alkaline phosphatase
  • Apoptosis
  • Ascorbic acid
  • Cell death
  • Mouse embryonic stem cells
  • Necrosis
  • Oxidative stress
  • Paraquat
  • Pesticide
  • Reactive oxygen species
  • Vitamin C

ASJC Scopus subject areas

  • Toxicology

Cite this

Paraquat toxicity in a mouse embryonic stem cell model. / Perla, Venu; Perrin, Nancy A; Greenlee, Anne R.

In: Toxicology in Vitro, Vol. 22, No. 2, 03.2008, p. 515-524.

Research output: Contribution to journalArticle

Perla, Venu ; Perrin, Nancy A ; Greenlee, Anne R. / Paraquat toxicity in a mouse embryonic stem cell model. In: Toxicology in Vitro. 2008 ; Vol. 22, No. 2. pp. 515-524.
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