Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA)

Wei Shao, Hua Jiang Wei, Ji Ying Qiao, Yong Chao Zhao, Yi Min Sun, Yu Xiang Zhou, Jing Cheng

Research output: Contribution to journalArticlepeer-review

Abstract

Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data. This method could simplify the assay of multiple TFs and may facilitate high-throughput profiling of large numbers of TFs.

Original languageEnglish (US)
Pages (from-to)1451-1456
Number of pages6
JournalJournal of proteome research
Volume4
Issue number4
DOIs
StatePublished - Jul 1 2005
Externally publishedYes

Keywords

  • DNA-binding activity
  • EMSA
  • Oligonucleotide array
  • Oligonucleotide array-based transcription factor assay (OATFA)
  • Transcription factor

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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