Papillomavirus L1 capsids agglutinate mouse erythrocytes through a proteinaceous receptor

Richard B.S. Roden, Nancy L. Hubbert, Reinhard Kirnbauer, Françoise Breitburd, Douglas R. Lowy, John T. Schiller

Research output: Contribution to journalComment/debatepeer-review

Abstract

Virus-like particles (VLPs) composed of L1 derived from bovine papillomavirus type 1 (BPV-1), several human papillomavirus types, or cottontail rabbit papillomavirus (CRPV) agglutinated mouse but not human or rat erythrocytes. Treatment of mouse erythrocytes with trypsin prevented hemagglutination (HA) by BPV-L Sera from rabbits immunized with native CRPV VLPs, which protect against experimental CRPV infection, exhibited high titers of antibodies that inhibited CRPV VLP HA activity, while sera from rabbits immunized with denatured CRPV VLPs or native BPV VLPs, which do not protect against CRPV infection, were not inhibitory. Testing for HA inhibition is a rapid and simple method for examining the serological relatedness of papillomaviruses and measuring protective antibody titers after VLP vaccination.

Original languageEnglish (US)
Pages (from-to)5147-5151
Number of pages5
JournalJournal of virology
Volume69
Issue number8
DOIs
StatePublished - Aug 1995
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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