@article{4c656c72524d40eca55b5e9367abd8a1,
title = "Pancreatic regeneration (reg) gene expression in a rat model of islet hyperplasia",
abstract = "Background. After pancreatectomy, regeneration of acinar and islet elements occurs. Recent data from a model of islet hyperplasia in the hamster suggested that induction of a local pancreatic factor stimulates islet formation. We postulate that the reg gene may be this factor. Methods. We studied reg expression during induction of islet growth by, using a similar model in the rat. Rats underwent surgical wrapping of the splenic lobe of the pancreas or sham operation. Results. At 2 days ductular proliferation and immunohistochemical evidence of insulin within ductular epithelia were evident in the wrapped lobe. By 14 and 56 days islet number per square millimeter increased 63% and 43%, respectively. Reg mRNA levels, measured by Northern blot analysis with a rat reg cDNA probe (n = 5), increased 300% at 2 days in the wrapped lobe and decreased to that of unwrapped controls by 14 days. In situ hybridization showed localization of reg to the acinar cells. In unwrapped gastric lobes of animals who underwent surgical wrapping of the splenic lobe, no change in islet number per square millimeter or reg gene expression was noted. Conclusions. Surgical wrapping of the pancreatic splenic lobe induces local reg gene expression that is temporally associated with duct cell hyperplasia. This is followed by islet formation within the wrapped lobe. Reg may play a role in the induction of new islets from ductular precursors and in maintenance of normal islet function.",
author = "Zenilman, {M. E.} and R. Perfetti and K. Swinson and T. Magnuson and Shuldiner, {A. R.}",
note = "Funding Information: ISLETS OF LANGERHANS are able to regenerate after subtotal pancreatic resection or destruction by pancreatit-is.I, 2 Analysis of pancreatic parenchyma in rodents undergoing such regeneration has revealed a marked increase in the expression of pancreatic regenerating (reg) gene. 2 Rat reg is expressed in acinar cells and possibly regenerating islets. 24 The rat reggene is homologous to the reg I gene of the mouse, a member of a family of three proteins related to calcium-dependent (C-type) lectins 5 that have known growth-promoting properties. The role of regin islet regeneration, however, is controversial; although recombinant rat reg protein exogenously administered after pancreatectomy has been shown to be trophic to islet \[3-cells6, others contend that rat reg gene expression does not correlate with \[3-cell growth. 7 Supported by an American College of Surgeons Faculty Fellowship (M.E.Z.) and Chesapeake Education and Research Trust (M.E.Z. and T.H.M.). Accepted for publication Oct. 4, 1995. Reprint requests: Michael E. Zenilman, MD, Department of Surgery, Albert Einstein College of Medicine, Montefiore Medical Park, Suite 125, 1575 Blondell Ave., Bronx, NY 10461. 11/56/70161 We studied reggene expression in a model of islet hyperplasia (nesidioblastosis) after nonocclusive pancreatic wrapping. In the hamster this preparation causes ductal hypertension and ductular proliferation within 2 weeks. Eight weeks after wrapping, new islets are visible in the wrapped lobe. 8' 9 Experiments have suggested that a growth factor produced early within the parenchyma of these regenerating pancreata acts to stimulate new islet cell formation. 1~ 11 Because the reg gene sequence of the hamster is unknown, quantitative determination of its role in this model has been difficulL 12'13 We hypothesized that in the wrapped pancreas, reg indeed might be involved in the formation of new islets, and if an homologous regprobe was used, regexpression would be shown to increase during islet formation. Because the sequence of rat regis known, 2 we characterized the expression of the reggene in the rat during islet formation induced by pancreatic wrapping. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",
year = "1996",
doi = "10.1016/S0039-6060(96)80270-4",
language = "English (US)",
volume = "119",
pages = "576--584",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "5",
}