Pancreatic cancer associated fibroblasts display normal allelotypes

Kimberly Walter, Noriyuki Omura, Seung Mo Hong, Margaret Griffith, Michael S Goggins

Research output: Contribution to journalArticle

Abstract

Background: Recent studies have reported widespread copy number alterations and p53 mutations arising in cancer associated stromal cells. The aim of this study was to determine if pancreatic cancer associated fibroblasts display similar genetic alterations. Design: Cancer-associated fibroblast cultures were established from 7 primary pancreatic adenocarcinomas. These fibroblasts and corresponding normal tissues when available were analyzed for genome-wide copy number changes using Affymetrix 250K SNP microarrays. Evidence of p53 protein expression, an indicator of p53 mutation was determined by immunohistochemical labeling of tissue microarrays containing 117 pancreatic ductal adenocarcinomas. Results: Pancreatic cancer associated fibroblasts did not show any evidence of somatic copy number gains or losses. p53 protein expression was confined to invasive pancreatic adenocarcinoma cells and was not expressed in cancer-associated fibroblasts. Conclusions: We find no evidence that pancreatic cancer associated fibroblasts harbor somatic copy number changes or immunohistochemical evidence of p53 mutations.

Original languageEnglish (US)
Pages (from-to)882-888
Number of pages7
JournalCancer Biology and Therapy
Volume7
Issue number6
DOIs
StatePublished - Jun 2008

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Keywords

  • Fibroblast
  • LOH
  • Microarray
  • p53
  • Pancreatic cancer
  • SNP

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Molecular Medicine
  • Pharmacology
  • Medicine(all)

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