TY - JOUR
T1 - Paired-recordings from synaptically coupled cortical and hippocampal neurons in acute and cultured brain slices
AU - Debanne, Dominique
AU - Boudkkazi, Sami
AU - Campanac, Emilie
AU - Cudmore, Robert H.
AU - Giraud, Pierre
AU - Fronzaroli-Molinieres, Laure
AU - Carlier, Edmond
AU - Caillard, Olivier
N1 - Funding Information:
ACKNOWLEDGMENTS We thank M. Seagar, O. El Far, J.M. Goaillard, Y. Frégnac and G. Alcaraz for stimulating discussions and F. Dubruc for help with video recordings. This work was supported by the Institut National de la Santé et de la Recherche Médicale, the Centre National de la Recherche Scientifique, the Agence Nationale de la Recherche (Neuroscience, Neurologie and Psychiatrie, 06-Neuro-014-01 to D.D.), the Fondation pour la Recherche Médicale (to E.C.), the Fondation Franc¸aise pour la Recherche sur l’Epilepsie (to O.C.), the Ministry of Research (doctoral grant to S.B. & E.C. and ACI Jeunes Chercheurs to D.D.), AFM (to R.C.) and the European Community (LSHM-CT-2004-511995, synaptic scaffolding proteins orchestrating cortical synapse organization during development to D.D.).
PY - 2008
Y1 - 2008
N2 - Analysis of synaptic transmission, synaptic plasticity, axonal processing, synaptic timing or electrical coupling requires the simultaneous recording of both the pre- and postsynaptic compartments. Paired-recording technique of monosynaptically connected neurons is also an appropriate technique to probe the function of small molecules (calcium buffers, peptides or small proteins) at presynaptic terminals that are too small to allow direct whole-cell patch-clamp recording. We describe here a protocol for obtaining, in acute and cultured slices, synaptically connected pairs of cortical and hippocampal neurons, with a reasonably high probability. The protocol includes four main stages (acute/cultured slice preparation, visualization, recording and analysis) and can be completed in ∼4 h.
AB - Analysis of synaptic transmission, synaptic plasticity, axonal processing, synaptic timing or electrical coupling requires the simultaneous recording of both the pre- and postsynaptic compartments. Paired-recording technique of monosynaptically connected neurons is also an appropriate technique to probe the function of small molecules (calcium buffers, peptides or small proteins) at presynaptic terminals that are too small to allow direct whole-cell patch-clamp recording. We describe here a protocol for obtaining, in acute and cultured slices, synaptically connected pairs of cortical and hippocampal neurons, with a reasonably high probability. The protocol includes four main stages (acute/cultured slice preparation, visualization, recording and analysis) and can be completed in ∼4 h.
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U2 - 10.1038/nprot.2008.147
DO - 10.1038/nprot.2008.147
M3 - Article
C2 - 18802437
AN - SCOPUS:52749095161
SN - 1754-2189
VL - 3
SP - 1559
EP - 1568
JO - Nature protocols
JF - Nature protocols
IS - 10
ER -