PABA: A PET tracer targeting bacteria-specific metabolism

Christopher Mutch, Alvaro Ordonez Suarez, Hecong Qin, Matthew F.L. Parker, Lauren E. Bambarger, Javier Villanueva-Meyer, Joseph E. Blecha, Valerie Carroll, Céline Taglang, Robert R. Flavell, Renuka Sriram, Henry F. Vanbrocklin, Oren Rosenberg, Michael Ohliger, Sanjay Jain, Kiel Neumann, David Wilson

Research output: Contribution to journalArticle

Abstract

Many methods to diagnose bacterial infections rely on imaging. These techniques include computed tomography (CT) and magnetic resonance imaging (MRI) for structural information, and positron emission tomography (PET) for metabolic data. However, frequently there is significant overlap in the imaging appearance of infectious and non-infectious entities using these tools. To address this concern, recent approaches have targeted bacteria-specific metabolic pathways. For example, radiolabelled sugars derived from sorbitol and maltose have been investigated as PET radiotracers, since these are efficiently incorporated into bacteria but are poor substrates for mammalian cells. We have previously shown that para-aminobenzoic acid (PABA) is an excellent candidate for development as a bacteria-specific imaging tracer as it is rapidly accumulated by a wide range of pathogenic bacteria, including metabolically quiescent bacteria and clinical strains, but not by mammalian cells. Therefore, in this study we developed an efficient radiosynthesis for [11C]PABA, investigated its accumulation into Escherichia coli and Staphylococcus aureus laboratory strains in vitro, and shown that it can distinguish between infection and sterile inflammation in a murine model of acute bacterial infection.

Original languageEnglish (US)
JournalUnknown Journal
DOIs
StateAccepted/In press - Mar 11 2018

Fingerprint

4-Aminobenzoic Acid
Positron-Emission Tomography
targeting
tomography
metabolism
tracer
Bacteria
bacterium
acid
Bacterial Infections
Maltose
Sorbitol
Metabolic Networks and Pathways
Staphylococcus aureus
sugar
Tomography
Magnetic Resonance Imaging
Escherichia coli
Inflammation
substrate

Keywords

  • bacteria
  • folate
  • Infection
  • metabolism
  • positron emission tomography

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Mutch, C., Ordonez Suarez, A., Qin, H., Parker, M. F. L., Bambarger, L. E., Villanueva-Meyer, J., ... Wilson, D. (Accepted/In press). PABA: A PET tracer targeting bacteria-specific metabolism. Unknown Journal. https://doi.org/10.1021/acsinfecdis.8b00061

PABA : A PET tracer targeting bacteria-specific metabolism. / Mutch, Christopher; Ordonez Suarez, Alvaro; Qin, Hecong; Parker, Matthew F.L.; Bambarger, Lauren E.; Villanueva-Meyer, Javier; Blecha, Joseph E.; Carroll, Valerie; Taglang, Céline; Flavell, Robert R.; Sriram, Renuka; Vanbrocklin, Henry F.; Rosenberg, Oren; Ohliger, Michael; Jain, Sanjay; Neumann, Kiel; Wilson, David.

In: Unknown Journal, 11.03.2018.

Research output: Contribution to journalArticle

Mutch, C, Ordonez Suarez, A, Qin, H, Parker, MFL, Bambarger, LE, Villanueva-Meyer, J, Blecha, JE, Carroll, V, Taglang, C, Flavell, RR, Sriram, R, Vanbrocklin, HF, Rosenberg, O, Ohliger, M, Jain, S, Neumann, K & Wilson, D 2018, 'PABA: A PET tracer targeting bacteria-specific metabolism', Unknown Journal. https://doi.org/10.1021/acsinfecdis.8b00061
Mutch, Christopher ; Ordonez Suarez, Alvaro ; Qin, Hecong ; Parker, Matthew F.L. ; Bambarger, Lauren E. ; Villanueva-Meyer, Javier ; Blecha, Joseph E. ; Carroll, Valerie ; Taglang, Céline ; Flavell, Robert R. ; Sriram, Renuka ; Vanbrocklin, Henry F. ; Rosenberg, Oren ; Ohliger, Michael ; Jain, Sanjay ; Neumann, Kiel ; Wilson, David. / PABA : A PET tracer targeting bacteria-specific metabolism. In: Unknown Journal. 2018.
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