p63 (TP73L) a key player in embryonic urogential development with significant dysregulation in human bladder exstrophy tissue

Bonnie J. Ching, Lars Wittler, Judith Proske, Garima Yagnik, Lihong Qi, Markus Draaken, Heiko Reutter, John Phillip Gearhart, Michael Ludwig, Simeon A. Boyadjiev

Research output: Contribution to journalArticle

Abstract

Human bladder exstrophy-epispadias complex (BEEC) comprises a spectrum of urogenital anomalies in which part or all of the distal urinary tract fails to close. Several lines of evidence implicate genetic factors in the formation of BEEC. Among them a murine p63-/- knockout model showed the full picture of classic exstrophy of the bladder and other urogenital defects within the BEEC spectrum. This led us to study in depth the role of p63 in urogenital development in mice and investigate the implication of p63 in human BEEC. Whole mount in situ analysis in mice was carried out to investigate the ventro-caudal expression of the p63 transcript at gestational days (GD) 9.5-12.5, the equivalent of human gestational weeks 4-6 (postulated time of BEEC organogenesis in humans). In addition, p63 expression analysis was performed in human blood and bladder derived samples of 15 BEEC newborns accompanied by sequencing analysis of their genomic DNA. We also conducted sequencing analysis of genomic DNA in additional 22 BEEC patients. In mouse embryos, p63 expression was detected at days 9.5-12.5 in the cloacal membrane and urethral epithelium, supporting its role in the morphogenesis of the external genitalia and the bladder. Tissue-specific expression of a novel and already-known mRNA isoforms were established and a reproducible dysregulation of variable p63 isoforms was observed in 11 of 15 patients indicating altered gene expression. However, no obvious p63 gene mutations were identified in any of the patients. Our findings strongly suggest that p63 is not only involved in embryonic formation of the urogenital and ventrocaudal anatomy but is also highly dysregulated in human BEEC bladder tissue. Since p63 has been shown to self-regulate its expression through a balance of its isoforms, the dysregulation observed may contribute to the formation of BEEC.

Original languageEnglish (US)
Pages (from-to)861-867
Number of pages7
JournalInternational Journal of Molecular Medicine
Volume26
Issue number6
DOIs
StatePublished - Dec 2010

Fingerprint

Bladder Exstrophy
Embryonic Development
Urinary Bladder
Protein Isoforms
RNA Isoforms
Bladder Exstrophy and Epispadias Complex
Genitalia
Organogenesis
DNA
Urinary Tract
Morphogenesis
Anatomy
Embryonic Structures
Epithelium
Newborn Infant
Gene Expression

Keywords

  • Bladder exstrophy
  • Epispadias
  • Expression analysis
  • P63
  • TP73L
  • Whole mount in situ expression

ASJC Scopus subject areas

  • Genetics

Cite this

p63 (TP73L) a key player in embryonic urogential development with significant dysregulation in human bladder exstrophy tissue. / Ching, Bonnie J.; Wittler, Lars; Proske, Judith; Yagnik, Garima; Qi, Lihong; Draaken, Markus; Reutter, Heiko; Gearhart, John Phillip; Ludwig, Michael; Boyadjiev, Simeon A.

In: International Journal of Molecular Medicine, Vol. 26, No. 6, 12.2010, p. 861-867.

Research output: Contribution to journalArticle

Ching, Bonnie J. ; Wittler, Lars ; Proske, Judith ; Yagnik, Garima ; Qi, Lihong ; Draaken, Markus ; Reutter, Heiko ; Gearhart, John Phillip ; Ludwig, Michael ; Boyadjiev, Simeon A. / p63 (TP73L) a key player in embryonic urogential development with significant dysregulation in human bladder exstrophy tissue. In: International Journal of Molecular Medicine. 2010 ; Vol. 26, No. 6. pp. 861-867.
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abstract = "Human bladder exstrophy-epispadias complex (BEEC) comprises a spectrum of urogenital anomalies in which part or all of the distal urinary tract fails to close. Several lines of evidence implicate genetic factors in the formation of BEEC. Among them a murine p63-/- knockout model showed the full picture of classic exstrophy of the bladder and other urogenital defects within the BEEC spectrum. This led us to study in depth the role of p63 in urogenital development in mice and investigate the implication of p63 in human BEEC. Whole mount in situ analysis in mice was carried out to investigate the ventro-caudal expression of the p63 transcript at gestational days (GD) 9.5-12.5, the equivalent of human gestational weeks 4-6 (postulated time of BEEC organogenesis in humans). In addition, p63 expression analysis was performed in human blood and bladder derived samples of 15 BEEC newborns accompanied by sequencing analysis of their genomic DNA. We also conducted sequencing analysis of genomic DNA in additional 22 BEEC patients. In mouse embryos, p63 expression was detected at days 9.5-12.5 in the cloacal membrane and urethral epithelium, supporting its role in the morphogenesis of the external genitalia and the bladder. Tissue-specific expression of a novel and already-known mRNA isoforms were established and a reproducible dysregulation of variable p63 isoforms was observed in 11 of 15 patients indicating altered gene expression. However, no obvious p63 gene mutations were identified in any of the patients. Our findings strongly suggest that p63 is not only involved in embryonic formation of the urogenital and ventrocaudal anatomy but is also highly dysregulated in human BEEC bladder tissue. Since p63 has been shown to self-regulate its expression through a balance of its isoforms, the dysregulation observed may contribute to the formation of BEEC.",
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