p53 Represses Transcription of RING Finger LIM Domain-Binding Protein RLIM through Sp1

Xiangtao Kong, Bo Peng, Yang Yang, Pingzhao Zhang, Bo Qin, Dingding Han, Chenji Wang, Yongjun Dang, Jun Liu, Long Yu

Research output: Contribution to journalArticle

Abstract

RLIM acts as a negative regulator of LIM-Homeodomain proteins either by recruiting Sin3A/Histone Deacetylase (HDAC) co-repressor complex or through degradation of CLIM coactivator, thus playing an important role in embryonic development. Recent studies by different research groups have shown that RLIM acts as an X-encoded, dose-dependent inducer of X chromosome inactivation in mouse embryonic stem cells. However, until now, very little is known about the expression regulation of RLIM gene, and we tried to study the transctriptional regulation of RLIM gene. In the present study, we identified RLIM as a novel target of p53 and demonstrated that p53 repressed both mRNA and protein levels of RLIM. Expression of wild type p53, but not p53 mutants, led to repression of the RLIM promoter activity. We further identified four putative Sp1 elements (S1 to S4) on the RLIM promoter that are essential for p53-mediated repression of RLIM. Although p53 does not directly bind to the RLIM promoter, it physically interacts with and prevents the binding of Sp1 to the RLIM promoter. Thus, RLIM is a novel target of p53, and p53 exerts its inhibitory effect on RLIM expression by interfering with Sp1-mediated transcriptional activation on RLIM. Our results provided data to enlarge the knowledge of transcriptional regulation of RLIM and suggested a new pathway by which physiological and pathological activators of p53 may affect development.

Original languageEnglish (US)
Article numbere62832
JournalPLoS One
Volume8
Issue number5
DOIs
StatePublished - May 1 2013

Fingerprint

LIM-Homeodomain Proteins
LIM Domain Proteins
RING Finger Domains
X Chromosome Inactivation
Co-Repressor Proteins
Histone Deacetylases
Gene Expression Regulation
Transcription
Transcriptional Activation
Embryonic Development
binding proteins
Carrier Proteins
transcription (genetics)
Genes
promoter regions
Messenger RNA
Chromosomes
Stem cells
Research
Proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Kong, X., Peng, B., Yang, Y., Zhang, P., Qin, B., Han, D., ... Yu, L. (2013). p53 Represses Transcription of RING Finger LIM Domain-Binding Protein RLIM through Sp1. PLoS One, 8(5), [e62832]. https://doi.org/10.1371/journal.pone.0062832

p53 Represses Transcription of RING Finger LIM Domain-Binding Protein RLIM through Sp1. / Kong, Xiangtao; Peng, Bo; Yang, Yang; Zhang, Pingzhao; Qin, Bo; Han, Dingding; Wang, Chenji; Dang, Yongjun; Liu, Jun; Yu, Long.

In: PLoS One, Vol. 8, No. 5, e62832, 01.05.2013.

Research output: Contribution to journalArticle

Kong, X, Peng, B, Yang, Y, Zhang, P, Qin, B, Han, D, Wang, C, Dang, Y, Liu, J & Yu, L 2013, 'p53 Represses Transcription of RING Finger LIM Domain-Binding Protein RLIM through Sp1', PLoS One, vol. 8, no. 5, e62832. https://doi.org/10.1371/journal.pone.0062832
Kong, Xiangtao ; Peng, Bo ; Yang, Yang ; Zhang, Pingzhao ; Qin, Bo ; Han, Dingding ; Wang, Chenji ; Dang, Yongjun ; Liu, Jun ; Yu, Long. / p53 Represses Transcription of RING Finger LIM Domain-Binding Protein RLIM through Sp1. In: PLoS One. 2013 ; Vol. 8, No. 5.
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