p53 Mutation and MDM2 Amplification in Human Soft Tissue Sarcomas

Fredrick S. Leach, Takashi Tokino, Jonathan D. Oliner, David Sidransky, Kenneth W. Kinzler, Bert Vogelstein

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Abstract

The p53 and MDM2 genes were analyzed in 24 human soft tissue sarcomas (11 malignant fibrous histiocytomas and 13 liposarcomas). Alterations of p53, consisting of point mutations, deletions, or overexpression, were detected in one-third (8 of 24) of the sarcomas. MDM2 gene amplification was detected in another 8 tumors, but no tumor contained an alteration of both genes. Monoclonal antibodies reactive with the human MDM2 gene product were developed, and immunohistochemical analysis revealed nuclear localization and overexpression of MDM2 in those tumors with amplified MDM2 genes. These data support the hypothesis that pS3 and MDM2 genetic alterations are alternative mechanisms for inactivating the same regulatory pathway for suppressing cell growth.

Original languageEnglish (US)
Pages (from-to)2231-2234
Number of pages4
JournalCancer Research
Volume53
Issue number10
StatePublished - May 1993

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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