p53-mediated repression of nuclear factor-κB RelA via the transcriptional integrator p300

Rajani Ravi, Bijoyesh Mookerjee, Yvette Van Hensbergen, Gauri C. Bedi, Antonio Giordano, Wafik S. El-Deiry, Ephraim J Fuchs, Atul Bedi

Research output: Contribution to journalArticle

Abstract

The p53 tumor suppressor gene plays an instrumental role in transcriptional regulation of target genes involved in cellular stress responses. p53-dependent transactivation and transrepression require its interaction with p300/CBP, a coactivator that also interacts with the ReIA subunit of nuclear factor-κB. We find that p53 inhibits RelA-dependent transactivation without altering ReIA expression or inducible κB-DNA binding. p53-mediated repression of RelA is relieved by p300 overexpression and the increased RelA activity conferred by p53-deficiency is counteracted by either transactivation domain-deficient p300 fragments that bind RelA or a transdominant mutant of IκBα. Our results suggest that p53 can regulate diverse κB-dependent cellular responses.

Original languageEnglish (US)
Pages (from-to)4531-4536
Number of pages6
JournalCancer Research
Volume58
Issue number20
StatePublished - Oct 15 1998

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Transcriptional Activation
p300-CBP Transcription Factors
B-Form DNA
Tumor Suppressor Genes
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ravi, R., Mookerjee, B., Van Hensbergen, Y., Bedi, G. C., Giordano, A., El-Deiry, W. S., ... Bedi, A. (1998). p53-mediated repression of nuclear factor-κB RelA via the transcriptional integrator p300. Cancer Research, 58(20), 4531-4536.

p53-mediated repression of nuclear factor-κB RelA via the transcriptional integrator p300. / Ravi, Rajani; Mookerjee, Bijoyesh; Van Hensbergen, Yvette; Bedi, Gauri C.; Giordano, Antonio; El-Deiry, Wafik S.; Fuchs, Ephraim J; Bedi, Atul.

In: Cancer Research, Vol. 58, No. 20, 15.10.1998, p. 4531-4536.

Research output: Contribution to journalArticle

Ravi, R, Mookerjee, B, Van Hensbergen, Y, Bedi, GC, Giordano, A, El-Deiry, WS, Fuchs, EJ & Bedi, A 1998, 'p53-mediated repression of nuclear factor-κB RelA via the transcriptional integrator p300', Cancer Research, vol. 58, no. 20, pp. 4531-4536.
Ravi R, Mookerjee B, Van Hensbergen Y, Bedi GC, Giordano A, El-Deiry WS et al. p53-mediated repression of nuclear factor-κB RelA via the transcriptional integrator p300. Cancer Research. 1998 Oct 15;58(20):4531-4536.
Ravi, Rajani ; Mookerjee, Bijoyesh ; Van Hensbergen, Yvette ; Bedi, Gauri C. ; Giordano, Antonio ; El-Deiry, Wafik S. ; Fuchs, Ephraim J ; Bedi, Atul. / p53-mediated repression of nuclear factor-κB RelA via the transcriptional integrator p300. In: Cancer Research. 1998 ; Vol. 58, No. 20. pp. 4531-4536.
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